Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/10061
Title: Microgravity as a means to incorporate HepG2 aggregates in polysaccharide-protein hybrid scaffold
Authors: Sarika, PR
James, NR
Anilkumar, PR
Raj, DK
Kumary, TV
Keywords: Engineering; Materials Science
Issue Date: 2016
Publisher: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Citation: 27 ,2;-
Abstract: Tissue culture under microgravity provides a venue which promotes cell-cell association while avoiding the detrimental effects of high shear stress. Hepatocytes cultured on carriers or entrapped within matrices under simulated microgravity conditions showed improved cell function and proliferation. In the present study, a new approach was adopted where a non-cell adherent scaffold was incorporated with hepatospheroids (HepG2) under microgravity. Gum arabic (GA) was cross-linked with gelatin (GA-Gel) and collagen (GA-Col) to prepare non-cell adherent scaffolds. Microgravity experiments with GA-Gel and GA-Col indicated that GA-Col is a better substrate compared to GA-Gel. Microgravity experiments of GA-Col scaffolds with HepG2 cells confirmed that the non-adherent surface with porous architecture can incorporate hepatocyte spheroids and maintain liver specific functions. Albumin and urea synthesis of hepatocytes was sustained up to 6 days under microgravity conditions in the presence of GA-Col scaffold. This new approach of using non-cell adherent matrix and microgravity environment for developing biological substitutes will be beneficial in tissue engineering, bioartificial liver devices and in vitro safety assessment of drugs.
URI: 10.1007/s10856-015-5638-5
http://dspace.sctimst.ac.in/jspui/handle/123456789/10061
Appears in Collections:Journal Articles

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