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Title: Rapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections
Authors: Kumar, BS
Sandhyamani, S
Nazeer, SS
Jayasree, RS
Keywords: Biochemistry & Molecular Biology; Biophysics
Issue Date: 2015
Citation: 52 ,1;107-110
Abstract: Autofluorescence exhibited by tissues often interferes with immunofluorescence. Using imaging and spectral analysis, we observed remarkable reduction of autofluorescence of formalin fixed paraffin embedded tissues irradiated with light prior to incubation with immunofluorescent dyes. The technique of photobleaching offers significant improvement in the quality and specificity of immunofluorescence. This has the potential for better techniques for disease diagnosis.
Appears in Collections:Journal Articles

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