Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/1066
Title: Spermine grafted galactosylated chitosan for improved nanoparticle mediated gene delivery
Authors: Alex, Susan M.
Rekha, M. R.
Sharma, Chandra P.
Keywords: Drug Delivery
Issue Date: 2011
Publisher: INTERNATIONAL JOURNAL OF PHARMACEUTICS
Citation: INTERNATIONAL JOURNAL OF PHARMACEUTICS. 410; 40910; 125-137
Abstract: Despite multitude of beneficial features, chitosan has poor water solubility and transfection ability which affect its gene delivery efficacy. The two features are improved when certain chemical modifications are incorporated into the chitosan parent backbone. This strategy is adopted here, by coupling galactose and spermine into the chitosan backbone. The conjugation was determined with FTIR and (1)H NMR and nanoparticle morphology was assessed by TEM and AFM techniques. Particle size, zeta potential, buffering capacity and DNA binding ability gave encouraging result of enhanced solubility and stability. In vitro studies of GCSM in HepG2 cell lines displayed low cytotoxicity and improved transfection. We also identified the preference of receptor mediated internalization for nanoparticles cellular uptake by treating with cellular uptake inhibitors. The results evidently led us to comprehend that galactosylated chitosan-g-spermine could be considered as a promising chitosan derivative for conducting nanoparticle mediated gene delivery. (C) 2011 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.ijpharm.2011.02.067
http://www.ncbi.nlm.nih.gov/pubmed/21396993
http://dspace.sctimst.ac.in/jspui/handle/123456789/1066
Appears in Collections:Journal Articles

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