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Title: In vitro interaction and biocompatibility of titanate nanotubes with microglial cells
Authors: Sruthi, S
Loiseau, A
Boudon, J
Sallem, F
Maurizi, L
Mohanan, PV
Lizardc, G
Millot, N
Keywords: Apoptosis; Lysosomal integrity; Microglial activation; Mitochondrial hyperpolarisation; Reactive oxygen species; Titanate nanotubes
Issue Date: Aug-2018
Publisher: Toxicology and Applied Pharmacology
Citation: Sruthi S Loiseau A, Boudon J, Sallem F, Maurizi L, Mohanan PV, Lizardc G, Millot N. In vitro interaction and biocompatibility of titanate nanotubes with microglial cells. Toxicology and Applied Pharmacology. 2018 Aug;353:74-86
Abstract: Titanate nanotubes (TiONts) are promising agents for biomedical applications. Microglial activation and associated oxidative burst are major challenges in drug delivery applications across the brain. Here, TiONts were designed for drug delivery systems by functionalizing them with (3-aminopropyl) triethoxysilane (APTES), their interactions and biocompatibility were studied in vitro using murine microglial BV-2 cells. TiONts-APTES exposure resulted in increased ROS production and transient mitochondrial hyperpolarization. However, there was no indication of microglial proliferation in BV-2 cells as suggested by cell cycle analysis and morphology evaluation. The endocytosis as well as passive diffusion mediated TiONts-APTES internalization were proved by transmission electron microscopy (TEM) with and without amiloride, an endocytosis inhibiting agent. In addition, the TiONts-APTES exhibited good biocompatibility on microglial BV-2 cells as revealed by the plasma membrane integrity, lysosmal membrane integrity, morphology and viability analysis.
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