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Title: Effect of Surface Modified Fullerene C70 on the ROS Production and Cellular Integrity Using Chinese Hamster Ovarian Cells
Authors: Anju, S
Ashtami, J
Mohanan, PV
Keywords: ROS fullerene-C70 cellular integrity mitochondrial membrane potential flow cytometry apoptosis cytotoxi-city
Issue Date: Nov-2019
Publisher: General Chemistry
Citation: Anju S, Ashtami J, Mohanan PV. Effect of Surface Modified Fullerene C70 on the ROS Production and Cellular Integrity Using Chinese Hamster Ovarian Cells. General Chemistry. 2019; 5, 190022.
Abstract: Fullerene, a distinct buckyball structured carbon allotrope, has immense popularity among various scientific disciplines. Comparing various fullerene allotropes, recent attention is focused on C70 fullerene owing to it potent applications in various interdisciplinary arenas including optoelectronics, photovoltaics as well as in biomedical technology. Although C70 suggests as a tunable material in biomedicine, con-vincing results about its toxic effects are still under controversy, which recommends the necessity for proper toxicity evaluation. Dextran polysaccharide was effectively coated on C70 to overcome the poor dispersion in water and for achieving proper stabilization. The as-prepared material was further characterized using various sophisticated techniques such as DLS, TEM, FTIR and zeta potential. Chinese Hamster Ovarian cell lines (CHO) served as the subject for major experiments. Various cytotoxicity assays and DCFH-DA probe ROS scavenging analysis were done. Morphological examinations of major sub-cellular organelles were carried out with the aid of fluorescent microscopy for nuclear condensation, mitochondrial membrane potential, lysosomal integrity, cytoskeletal integrity, etc. Flow cytometric FACS analysis for possible apoptosis-necrosis mediated cell death assessment and DNA ladder experiment for genotoxicity was done. Cell viability assays show up to 80% live CHO cells after dextran-coated C70 treatment for 24 h. The fluorescent staining results that confirm intact or-ganelles further prove the non-toxic nature of dextran-coated C70. DNA laddering assay results ex-clude chances for genotoxic potential of dextran-coated C70. Experimental results of the present study indicate that dextran stabilized C70 fullerene is a potent candidate material for futuristic healthcare ap-plications.
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