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dc.contributorSumi, M G-
dc.contributorMathai, A-
dc.contributorReuben, S-
dc.contributorRadhakrishnan, V V-
dc.contributorSasikumar, S-
dc.contributorJayapal, V-
dc.contributorFelix, J-
dc.date.accessioned2012-12-04T11:43:11Z-
dc.date.available2012-12-04T11:43:11Z-
dc.date.issued2001-
dc.identifier.citationIndian journal of experimental biology. 39; 10; 984-8en_US
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/11883521-
dc.identifier.urihttp://dspace.sctimst.ac.in/jspui/handle/123456789/16-
dc.description.abstractIgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B. Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tuberculosis. With this mycobacterial antigen, a dot immunobinding assay (Dot-Iba) was developed for the detection of specific antibody to M. tuberculosis in the sera of patients with PT and controls. The assay gave positive results in all the 12 sputum-smear positive [acid fast bacilli (AFB)] patients with PT and gave negative results in the 50 sera from control groups. The Dot-Iba as described in this study, is simple, rapid and specific for laboratory diagnosis of PT.-
dc.publisherIndian journal of experimental biology-
dc.subjectLaboratory Diagnosis-
dc.titleA dot-immunobinding assay (dot-Iba) for rapid diagnosis of pulmonary tuberculosis.-
Appears in Collections:Journal Articles

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