Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/17
Title: A DOT-IMMUNOBINDING ASSAY FOR THE LABORATORY DIAGNOSIS OF TUBERCULOUS MENINGITIS AND ITS COMPARISON WITH ENZYME-LINKED-IMMUNOSORBENT-ASSAY
Authors: RADHAKRISHNAN, VV
MATHAI, A
Keywords: Laboratory Diagnosis
Issue Date: 1991
Publisher: JOURNAL OF APPLIED BACTERIOLOGY
Citation: JOURNAL OF APPLIED BACTERIOLOGY. 71; 5; 428-433
Abstract: In an attempt to establish an alternative to standard bacteriological methods in the laboratory diagnosis of tuberculous meningitis (TBM), a simple dot-immunobinding assay (Dot-Iba) was standardized to detect Mycobacterium tuberculosis antigen 5 and antimycobacterial antibody in cerebrospinal fluid (CSF) specimens of patients with TBM. Sensitivity and specificity of Dot-Iba was compared with conventional enzyme-linked immunosorbent assay (ELISA) and standard bacteriological techniques. The Dot-Iba showed excellent correlation with indirect ELISA for the detection of antimycobacterial antibody in CSF and showed 60% sensitivity and 100% specificity in culture-negative patients with TBM. However Dot-Iba was less sensitive for the detection of antigen 5 in CSFs and showed false negative results (60%) in culture-positive patients with TBM.
URI: http://dx.doi.org/10.1111/j.1365-2672.1991.tb03812.x
http://www.ncbi.nlm.nih.gov/pubmed/1761436
http://dspace.sctimst.ac.in/jspui/handle/123456789/17
Appears in Collections:Journal Articles

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