Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/215
Title: Cell mimetic monolayer supported chitosan-haemocompatibility studies
Authors: Mathews, Smitha
Kaladhar, K.
Sharma, Chandra P.
Keywords: Biocompatibility
Issue Date: 2006
Publisher: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Citation: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A. 79A; 1; 147-152
Abstract: Chitosan is a natural polymer, widely explored for biomedical and tissue engineering applications. However the thrombogenic nature limits their application in blood contacting devices and implants. Here, we have attempted to understand the haemocompatibility of chitosan by immobilizing a monolayer of cell mimetic lipid compositions. The phosphatidylcholine/cholesterol/galactocerebroside lipid composition (PC/Chol/GalC, 1:0.35:0.125) was deposited onto the chitosan films. Characterization of the modified surface was done by sessile drop contact angle measurement. The contact angle of the chitosan film reduced from 80.65 +/- 1.4 to 23.5 +/- 1.9 after the surface modification. Swelling nature of chitosan seemed to influence the orientation and packing of the lipid monolayer. In vitro calcification studies with metastable salt solution indicated increased calcification on the modified surface. This may be due to formation of nuclei for calcification on the expanding monolayer. The preliminary haemocompatibility studies with washed platelets, leukocytes and erythrocytes showed overall reduction in blood cell adhesion to the modified surfaces. Scanning electron microscopy was used for morphological characterization of platelet adhesion and activation on the surfaces. On the bare chitosan surface, fully spread platelets with extending pseudopodia indicated platelet activation. The smooth surface of the modified film did not activate platelets. These studies showed that, though the lipid monolayer on chitosan film is able to reduce the over all blood cell adhesion and platelet activation it is prone to calcification. (c) 2006 Wiley Periodicals, Inc.
URI: http://dx.doi.org/10.1002/jbm.a.30710
http://dspace.sctimst.ac.in/jspui/handle/123456789/215
Appears in Collections:Journal Articles

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.