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Title: Determination of antioxidant defense mechanism after acute oral administration of hydroxyapatite nanoparticles in rats.
Authors: Reshma, SC
Syama, S
Leji, B
Anju, M
Sreekanth, PJ
Varma, HK
Mohanan, PV
Keywords: Hydroxyapatite, nanoparticles, acute oral toxicity, oxidative stress, antioxidant enzyme haematology, biochemistry
Issue Date: Mar-2014
Publisher: J Free Rad Antioxidants
Citation: J Free Rad Antioxidants.. 2014;140:318- 27
Abstract: Hydroxyapatite nanoparticle (HANPs) has various applications like bone grafting, dental fillings, in toothpastes and mouth washes for teeth remineralization, etc. Even though HANPs application is a widely investigated area of research, the toxicity aspect still remains largely unexplored. This study focuses on the acute oral toxicity of HANPs exposed to Wistar rats, as its prospective application involve exposure via the oral route. In-house synthesized HANPs (<50 nm) was administered to rats orally. Hematological and biochemical parameters were carried out in blood from these animals. After sacrifice, gross necropsy was conducted and lipid peroxidation (LPO) and antioxidant status was estimated in the liver. During the experimental period, no HANPs related toxicity was observed and the hematological and biochemical parameters remained similar to control. However, dose dependent LPO in liver but the resultant oxidative stress was combated by the cells innate antioxidant defense mechanisms, which was evident with the increase in activity. Even though no death or adverse toxic effects were observed in the acute oral toxicity (unclassified compound as per Globally Harmonized System for Classification for chemical substances and mixtures) according to OECD 423, there was a dose dependant increase in the oxidative stress.
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