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Title: Constitution of FibrinBased Niche for In Vitro Differentiation of Adipose-Derived Mesenchymal Stem Cells to Keratinocytes
Authors: Unnikrishnan, S
Jayakumar, K
Krishnan, LK
Keywords: growth factor; stem cells; tissue engineering; wounds; regeneration
Issue Date: Apr-2015
Publisher: BioResearch Open Access
Citation: Unnikrishnan S, Jayakumar, K, Krishnan LK. Constitution of FibrinBased Niche for In Vitro Differentiation of Adipose-Derived Mesenchymal Stem Cells to Keratinocytes. BioResearch Open Access. 2014 Dec 1;3(6):339-47
Abstract: Epithelialization of chronic cutaneous wound is troublesome and may require use of skin/cell substitutes. Adipose- derived mesenchymal stem cells (ADMSCs) have immense potential as autologous cell source for treating wounds; they can cross the germ layer boundary of differentiation and regenerate skin. When multipotent adult stem cells are considered for skin regeneration, lineage committed keratinocytes may be beneficial to prevent undesirable post-transplantation outcome. This study hypothesized that ADMSCs may be directed to epidermal lineage in vitro on a specifically designed biomimetic and biodegradable niche. Cells were seeded on the test niche constituted with fibrin, fibronectin, gelatin, hyaluronic acid, laminin V, platelet growth factor, and epidermal growth factor in the presence of cell-specific differentiation medium (DM). The ADMSCs grown on bare tissue culture polystyrene surface in DM is designated DM-control and those grown in basal medium (BM) is the BM-control. Lineage commitment was monitored with keratinocyte-specific markers such as cytokeratin 14, cytokeratin 5, cytokeratin 19, and integrin a6 at the transcriptional/translational level. The in vitro designed biomimetic fibrin composite matrix may have potential application as cell transplantation vehicle.
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