Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/27
Title: A novel erythrocyte-based immunoassay for simultaneous detection of both antimycobacterial antibody response and mycobacterial antigen in human serum samples of pulmonary tuberculosis and a control group of patients using 'a single probe'
Authors: Katti, MK
Azeem, M
Keywords: Immunology
Issue Date: 2006
Publisher: FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
Citation: FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY. 47; 1; 134-137
Abstract: A modified passive hemagglutination using double aldehyde stabilized cells (tanned sheep erythrocytes treated with glutaraldehyde and pyruvic aldehyde) was evaluated for detection of both antimycobacterial antibodies and circulating mycobacterial antigens simultaneously in human serum samples from patients with pulmonary tuberculosis (n=40) and a control group (n=44). Double aldehyde stabilized cells sensitized with an optimum dose of 200 mu g mL(-1) of sonicate extract of Mycobacterium tuberculosis antigens was used as single probe to detect both antibodies and antigen, respectively, by passive hemagglutination and passive hemagglutination inhibition. The sensitivity limit of passive hemagglutination inhibition was determined to be 280 ng mL(-1) using a dose-response curve. Sensitivity of passive hemagglutination and passive hemagglutination inhibition, respectively, was 90% and 52.5%, and specificity was 91% and 100%. Although passive hemagglutination and passive hemagglutination inhibition need further evaluation, these erythrocyte-based immunoassays are potentially advantageous, especially as double aldehyde stabilized sensitized cells could be used as a single probe for detection of both antibodies and antigen. In addition, erythrocyte-based immunoassays are rapid, simple and cost-effective with a high degree of sensitivity.
URI: http://dx.doi.org/10.1111/j.1574-695X.2006.00074.x
http://dspace.sctimst.ac.in/jspui/handle/123456789/27
Appears in Collections:Journal Articles

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.