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Title: Induction of Cytotoxicity and Oxidative Stress of Dextran Coated Ferrite Nanoparticles (DFNPs) on A549 Cell Lines
Authors: Reshma, VG
Mohanan, PV
Keywords: Nanoparticles; Antioxidants; In vitro assays; Cytotoxicity; Genotoxicity; Biomedical
Issue Date: Dec-2016
Publisher: J of Pharmacol & Clin Res
Citation: Reshma VG, Mohanan PV. Induction of Cytotoxicity and Oxidative Stress of Dextran Coated Ferrite Nanoparticles (DFNPs) on A549 Cell Lines. J of Pharmacol & Clin Res. 2016;1(5).
Abstract: Super paramagnetic iron oxide nanoparticles have wide range of biomedical applications such as cellular and molecular imaging, site specific drug delivery etc. In the present study, in-house synthesized and characterized nano-size (<25nm) dextran coated ferrite nanoparticles (DFNPs) was subjected to evaluate the cytotoxic potential by using tetrazolium salts MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide using A549 cell lines (human lung carcinoma cell lines), a model of type 2 human alveolar epithelial cells. Similarly, the effects of DFNPs on oxidative stress (reduced glutathione, Glutathione reductase, glutathione peroxidase, superoxide dismutase and lipid peroxidase) using mice lung homogenate were also investigated. Results of the study pointed out that there was a slight fluctuation in antioxidant enzyme activity and lipid peroxidation, and was not significant when compared to control values. DFNPs did not show cytotoxicity up to a concentration of 100µg/ml. Hence, the present study can be concluded that the in-house synthesized DFNPs were non-cytotoxic and promising their use for biomedical applications.
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