In vitro release study and antimicorbial property evaluation of ofloxacin loaded poly (2-hydroxyethyl methacrylate)/poly (caprolactone)/poly (ethylene glycol) hydrogel system for burn wound management

Abstract

Monomer 2-hydroxy ethyl methacrylate containing small amounts of poly(caprolactone) and poly(ethylene glycol) incorporated with an antibiotic ofloxacin was polymerized by photo-polymerization technique using 2,4,6 trimethyl benzoyl diphenyl phosphine oxide (TPO) as photo-initiator. Encapsulation efficiency and in vitro drug release was studied using UV-visible spectroscopy. Swelling analysis was resorted to compare fluid uptake ability of hydrogel containing the drug with bare polymer. Zone of inhibition assay showed hydrogel containing 1% Ofloxacin to possess strong antimicrobial property Hemolysis assay demonstrated the hydrogel system to be non-hemolytic. Non-cytotoxic character of the hydrogel was confirmed using fibroblast cells. Cell adhesion studies showed non-attachment of fibroblasts to the polymer and improved cell proliferation simultaneously.