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Title: Curcumin Attenuates Glucose-Induced Monocyte Chemoattractant Protein-1 Synthesis in Aortic Endothelial Cells by Modulating the Nuclear Factor-kappa B Pathway
Authors: Panicker, Sumith Retnamma
Kartha, Chandrasekharan Cheranellore
Keywords: Cardiology
Issue Date: 2010
Citation: PHARMACOLOGY. 85; 1; 18-26
Abstract: Background/Aims: High glucose (HG) induces monocyte chemoattractant protein-1 (MCP-1) synthesis in endothelial cells through nuclear factor kappa B (NF kappa B). We investigated whether curcumin, losartan and sodium salicylate (NaSal) attenuate HG-induced MCP-1 synthesis in rat aortic endothelial cells (RAECs) and explored the mechanism of action. Methods: RAECs were stimulated with HG (25 mmol/l) for 24 h in the presence or absence of curcumin, losartan, NaSal or NF kappa B inhibitor, Bay 11-0782. The MCP-1 protein and mRNA levels were determined by enzyme-linked immunosorbent assay and real-time reverse transcriptase-polymerase chain reaction, respectively. Nuclear translocation of NF kappa B subunit p65 and NF kappa B DNA-binding activity was studied using confocal microscopy and electrophoretic mobility shift assay, respectively. Results: A significant increase in the synthesis of MCP-1 protein and mRNA (2-fold) was observed in HG-primed RAECs compared to control glucose (5.5 mmol/l). Curcumin (30 mu mol/l) significantly decreased HG-induced MCP-1 protein (74%) and mRNA (53%) synthesis. There was no inhibition of HG-induced MCP-1 protein secretion by losartan and NaSal. In HG-stimulated RAECs, curcumin attenuated the nuclear translocation of p65 and decreased the NF kappa B DNA-binding activity. Conclusion: Curcumin blocks HG-induced MCP-1 synthesis in RAECs partly via the NF kappa B pathway. Copyright (C) 2009 S. Karger AG, Basel
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