Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/353
Title: Dextran-glycidyltrimethylammonium chloride conjugate/DNA nanoplex: A potential non-viral and haemocompatible gene delivery system
Authors: Thomas, Jane Joy
Rekha, M. R.
Sharma, Chandra P.
Keywords: Drug Delivery
Issue Date: 2010
Publisher: INTERNATIONAL JOURNAL OF PHARMACEUTICS
Citation: INTERNATIONAL JOURNAL OF PHARMACEUTICS. 389; 40910; 195-206
Abstract: Non-viral gene carriers have attracted great interests for their unique properties. Cationic polymers have been in focus nowadays. Dextran is one of the most widely studied polymer in terms of gene therapy and in vivo disposition. But its applications are limited by its own drawbacks. To overcome the drawback, we have modified dextran using glycidyltrimethylammonium chloride (GTAC) bearing cationic groups. Nanoplexes were prepared using the derivative and calf thymus DNA (ctDNA) by reducing the surface charge and size of ctDNA. Complexation and stability of the nanoplex was proved using agarose gel electrophoresis and by Ethidium bromide (EtBr) displacement assay. Acid base titration studies were done to determine its buffering capacity. Derivatization was confirmed using NMR. Protection of ctDNA from nuclease digestion was evaluated. Stability of the nanoplex towards plasma components was analyzed. Its interactions with blood components were tested by haemolysis and aggregation studies. In vitro cytotoxicity studies have been done to investigate the effect of nanoplex on HepG2 cells by MU assay. This derivative has been proved to be feasible in transfection. The above investigations prove the capability of dextran modified with GTAC as a promising non-viral and haemocompatible gene delivery agent. (C) 2010 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.ijpharm.2010.01.011
http://www.ncbi.nlm.nih.gov/pubmed/20080161
http://dspace.sctimst.ac.in/jspui/handle/123456789/353
Appears in Collections:Journal Articles

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