Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/438
Title: Electroelution of lipoprotein(a) [Lp(a)] from native polyacrylamide gels: A new, simple method to purify Lp(a)
Authors: Chellan, Bijoy
Lttan, P. S. Appukuttan
Jayakumari, N.
Keywords: Biochemistry
Issue Date: 2006
Publisher: JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Citation: JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS. 68; 1; 43-53
Abstract: Lipoprotein(a), Lp(a), is an atherogenic lipoprotein consisting of an LDL like core particle and a covalently linked glycoprotein of variable size. Lp(a). isolated front serum always contains LDL and HDL, as contaminants since Lp(a) floats in the density range 1.05-1.12 g/ml which overlaps that of LDL and HDL, Purified Lp(a) is increasingly needed as a standard to overcome various problems in the standardization of Lp(a) measurements and for in vitro biological studies. Problems inherent to the purification of Lp(a) include the aggregation of Lp(a) with LDL, overlapping size distribution and the inability of some fractions to bind to affinity columns. Here. we describe the development of a new method to purify Lp(a) from contaminating LDL and HDL, particles. Lp(a) was isolated from serurn by sequential ultracentrifugation, resolved by native polyacrylamide eel electrophoresis and the eel segments were electroeluted to obtain pure Lp(a). L-Proline was added to the sample to a final concentration of 0.1 M to prevent the aggregation of Lp(a) with LDL. (c) 2006 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.jbbm.2006.03.012
http://www.ncbi.nlm.nih.gov/pubmed/16677712
http://dspace.sctimst.ac.in/jspui/handle/123456789/438
Appears in Collections:Journal Articles

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