Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/718
Title: Magnesium deficiency-related changes in lipid peroxidation and collagen metabolism in vivo in rat heart
Authors: Kumar, BP
Shivakumar, K
Kartha, CC
Keywords: Experimental Medicine
Issue Date: 1997
Publisher: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
Citation: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY. 29; 1; 129-134
Abstract: Magnesium deficiency is known to produce a cardiomyopathy, characterised by myocardial necrosis and fibrosis. As part of the ongoing investigations in this laboratory to establish the biochemical correlates of these histological changes, the present study probed the extent of lipid peroxidation and alterations in collagen metabolism in the heart in rats fed a magnesium-deficient diet for 28, 60 or 80 days. While lipid peroxidation was measured by the thiobarbituric acid reaction, collagen turnover rates and fibroblast proliferation were assessed using [H-3]-proline and [H-3]-thymidine, respectively. Tissue levels of magnesium and calcium were determined by atomic absorption spectrophotometry. A 39% increase in the cardiac tissue level of thiobarbituric acid reactive substances was observed on day 60 of deficiency (p < 0.001). A marked drop in collagen deposition rate (59%, p < 0.001%) on day 28 but a significant rise in fractional synthesis rate (12%, p < 0.001) and collagen deposition rate (24%, p < 0.001) on day 60 were observed. A fibroproliferative response in the heart was evident on day 80 but not at earlier time-points. Thus, the present study provides evidence of increased lipid peroxidation and net deposition of collagen in the myocardium in response to dietary deficiency of magnesium. These changes were, however, not directly related to alterations in the tissue levels of Mg. It is suggested that the increase in cardiac collagen synthesis and fibroplasia associated with Mg deficiency may represent reparative fibrogenesis, upon oxidative damage to the cardiac muscle, and is mediated by a mechanism independent of changes in cardiac tissue levels of Mg. (C) 1997 Elsevier Science Ltd.
URI: http://dx.doi.org/10.1016/S1357-2725(96)00124-0
http://dspace.sctimst.ac.in/jspui/handle/123456789/718
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