Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/845
Full metadata record
DC FieldValueLanguage
dc.contributorChacko, BK-
dc.contributorAppukuttan, PS-
dc.date.accessioned2012-12-04T11:44:49Z-
dc.date.available2012-12-04T11:44:49Z-
dc.date.issued2001-
dc.identifier.citationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 28; 5; 365-371en_US
dc.identifier.urihttp://dx.doi.org/10.1016/S0141-8130(01)00139-8-
dc.identifier.urihttp://dspace.sctimst.ac.in/jspui/handle/123456789/845-
dc.description.abstractPeanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour marker as it strongly recognises the cancer specific T antigen (Ga1 beta --> 3GalNAc-), but not its sialylated version. However, an additional specificity towards Gal beta1 --> 4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA. For correct interpretation of lectin histochemical results we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, rather than mono- or disaccharides as ligands. Dot-blots, transfer blots or polystyrene plate coatings of the soluble glycoconjugates were probed with horse-radish peroxidase (HRP) conjugates of PNA and other lectins of known specificity. Modifications of PNA-binding glycoproteins, including selective removal of O-linked oligosaccharides and treatment with glycosidases revealed that Gal beta1 --> 4GlcNAc (LacNAc) was ineffective while terminal alpha -linked galactose (TAG) as well as exposed T antigen (Gal beta1 --> 3 GalNAc-) was excellent as sugar moiety in glycoproteins for their recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Results were confirmed using TAG-specific human serum anti-a-galactoside antibody. (C) 2001 Elsevier Science B.V. All rights reserved.-
dc.publisherINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES-
dc.subjectBiochemistry-
dc.titlePeanut (Arachis hypogaea) lectin recognizes alpha-linked galactose, but not N-acetyl lactosamine in N-linked oligosaccharide terminals-
Appears in Collections:Journal Articles

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.