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|Title:||ApoB-INDEPENDENT ENZYME IMMUNOASSAY FOR LIPOPROTEIN(a) BY CAPTURE ON IMMOBILIZED LECTIN (JACALIN)|
|Keywords:||Biochemistry & Molecular Biology; Immunology; Medical Laboratory Technology|
|Publisher:||JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY|
|Abstract:||Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 +/- 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV=6.4%).|
|Appears in Collections:||Journal Articles|
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