Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/967
Title: RAPID ISOLATION OF HUMAN PLASMA ANTI-ALPHA-GALACTOSIDE ANTIBODY USING SUGAR-SPECIFIC BINDING TO GUAR GALACTOMANNAN OR AGAROSE
Authors: JAISON, PL
APPUKUTTAN, PS
Keywords: Biochemistry
Issue Date: 1992
Publisher: INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
Citation: INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS. 29; 3; 266-270
Abstract: A method of purifying the naturally occurring antibody to alpha-galactoside moiety (anti-alpha-Gal) in human plasma by a single-step affinity chromatography on cross-linked guar galactomannan (CLGG) or agarose (Sepharose 4B) is described. IgG nature of the two preparations, as revealed by agar gel diffusion, as well as their preference for alpha-anomer of galactose, as revealed in inhibition of their agglutination of trypsinized rabbit erythrocytes by sugars, identified them with anti-alpha-Gal. The antibody binding capacity of Sepharose 4B was only a third of that of CLGG. Both gels showed similar dependence on ionic strength for binding. The pH optimum for binding of anti-alpha-Gal to CLGG was 8.0. Significantly anti-alpha-Gal binding to Sepharose was unaffected by CNBr activation and ligand coupling to the gel, thus warning that contaminating plasma could introduce artifacts in agarose-based chromatography of human tissue biomolecules.
URI: http://dspace.sctimst.ac.in/jspui/handle/123456789/967
Appears in Collections:Journal Articles

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