Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/9724
Title: Evaluation of Polypropylene Hollow-Fiber Prototype Bioreactor for Bioartificial Liver
Authors: Palakkan, AA
Raj, DK
Rojan, J
Raj, RGS
Kumar, PRA
Muraleedharan, CV
Kumary, TV
Keywords: Cell Biology; Biotechnology & Applied Microbiology
Issue Date: 2013
Publisher: TISSUE ENGINEERING PART A
Citation: 19 ,43017;1056-1066
Abstract: Hepatocytes in high density are a requisite for the functional performance of complex devices such as bioartificial liver (BAL). In addition to high cell number, efficient mass transfer is also a key parameter in such devices. High-density culture of cells and efficient mass transfer can be achieved in BAL with hollow-fiber-based bioreactors. Even though different types of hollow fibers have been tried in a BAL, prospects of using polypropylene hollow fibers are not well evaluated. In this study, a prototype of bioreactor with polypropylene hollow fibers was fabricated and evaluated for cytotoxicity and hepatocyte function. High density of HepG2/adult hepatocyte cultures was used to evaluate polypropylene hollow fiber to support the biochemical activities (albumin and urea production), ammonia detoxification, and gene expression and to provide effective oxygenation. The results confirmed that a polypropylene hollow-fiber prototype bioreactor is able to provide efficient oxygenation and supported hepatocyte functions in a high-density culture.
URI: 10.1089/ten.tea.2012.0332
http://dspace.sctimst.ac.in/jspui/handle/123456789/9724
Appears in Collections:Journal Articles

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.