Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/9908
Title: In vitro binding of C-reactive protein on to chemically modified polymethyl methacrylate surfaces
Authors: Sreenivasan, K
Raj, V
Han, PR
Keywords: Chemistry; Electrochemistry; Instruments & Instrumentation
Issue Date: 2007
Publisher: SENSORS AND ACTUATORS B-CHEMICAL
Citation: 127 ,2;330-334
Abstract: Phosphorylethanolamine (PEA), C-reactive protein (CRP) specific ligand was coupled onto the surface of polymethyl methacrylate (PMMA) surface. Initially PMMA surface was modified by grafting glycidyl methacrylate (GMA). The anchoring of PEA on to PMMA was achieved through the ring opening reaction of epoxide group of GMA. The modified surface was characterized using FT-IR, scanning electron microscopy, energy dispersive X-ray analysis and contact angle measurement. The modified surface was found to bind CRP The adsorbed protein was visualized by staining Coomassie blue. The quantification of the extent of adsorption was carried out using spectrophotometric measurement. The methodology appears to be simple and expandable further for the development of a sensing element for CRP. (c) 2007 Elsevier B.V. All rights reserved.
URI: 10.1016/j.snb.2007.04.026
http://dspace.sctimst.ac.in/jspui/handle/123456789/9908
Appears in Collections:Journal Articles

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.