Please use this identifier to cite or link to this item: http://dspace.sctimst.ac.in/jspui/handle/123456789/9913
Title: In vitro evaluation of antioxidant defense mechanism and hemocompatibility of mauran
Authors: Raveendran, S
Palaninathan, V
Chauhan, N
Sakamoto, Y
Yoshida, Y
Maekawa, T
Mohanan, PV
Kumar, DS
Keywords: Chemistry; Polymer Science
Issue Date: 2013
Publisher: CARBOHYDRATE POLYMERS
Citation: 98 ,1;108-115
Abstract: Mauran (MR), a highly polyanionic sulfated exopolysaccharide was extracted from moderately halophilic bacterium; Halomonas maura and characterized using X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. Purified MR was evaluated for antioxidant defense mechanisms under in vitro conditions using L929, mouse fibroblast cell line and mice liver homogenate. It was demonstrated that MR could impart protective effect against oxidative stress in both cells and tissue up to a concentration of 500 mu g, which is found to be safe under laboratory conditions. Various enzymatic and non-enzymatic parameters of antioxidant mechanisms were evaluated and concluded that MR has the tendency to maintain a balance of antioxidative enzymes with in the test systems studied. Also, hemocompatibility assay performed revealed that MR has a lesser hemolytic index and exhibited a prolonged clotting time, which shows both antihemolytic, and antithrombogenic nature respectively. Furthermore, absorption studies performed using fluorescent-labeled MR confirmed that MR accumulated within the cell cytoplasm neither induced cellular lysis nor affected the cell integrity. (C) 2013 Elsevier Ltd. All rights reserved.
URI: 10.1016/j.carbpol.2013.04.050
http://dspace.sctimst.ac.in/jspui/handle/123456789/9913
Appears in Collections:Journal Articles

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.