Sumi, M GMathai, AReuben, SRadhakrishnan, V VSasikumar, SJayapal, VFelix, J2012-12-042012-12-042001Indian journal of experimental biology. 39; 10; 984-8http://www.ncbi.nlm.nih.gov/pubmed/11883521https://dspace.sctimst.ac.in/handle/123456789/16IgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B. Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tuberculosis. With this mycobacterial antigen, a dot immunobinding assay (Dot-Iba) was developed for the detection of specific antibody to M. tuberculosis in the sera of patients with PT and controls. The assay gave positive results in all the 12 sputum-smear positive [acid fast bacilli (AFB)] patients with PT and gave negative results in the 50 sera from control groups. The Dot-Iba as described in this study, is simple, rapid and specific for laboratory diagnosis of PT.Laboratory Diagnosis