Krishnan, VKLizymol, PPKumari, TVRauf, TAThomas, MM2017-03-102017-03-10200522 ,2;145-152https://dspace.sctimst.ac.in/handle/123456789/9565Development of a dentine bonding agent based on a tetramethacrylate resin with free carboxyl groups and its evaluation are described in this paper. Pyromellitic dianhydride was reacted with glycerol dimethactylate at 42-45 degrees C for 5 h in presence of an amine catalyst to produce a colorless and viscous pyromellitic anhydride-glycerol dimethacrylate adduct [PMGDM]. The resin was characterized using refractive index, infra red and NMR spectroscopy and HPLC chromatographic techniques. The resin was used to formulate a single solution bonding agent [SSBA] using acetone as the solvent, 2-hydroxyethyl methacrylate [HEMA] as the diluent, camphorquinone [CO] as the photoinitiator and dimethylamino phenethyl alcohol [DMAPEA] as the accelerator. Shear and tensile bond strengths of composites polymerized by photoinitiation adhered on dentinal and metallic surfaces using SSBA and an imported control Single Bond were evaluated and compared. Cytotoxicity evaluation by the MTT assay of SSBA and Single Bond on L-929 mammalian cell lines demonstrated metabolic activity of 1.7% of cells in contact with SSBA extract and 1.5% of cells in contact with Single Bond extract compared to 100% for cells alone. Shear and tensile strength evaluation at different intervals of time for the newly developed bonding agent stored at 22 +/- 2 degrees C upto 70 days showed no statistically significant variation in the strength values with time.Polymer Science