Syama, SAby, CPMaekawa, TSakthikumar, DMohanan, PV2017-06-272017-06-272017-05Syama S, Aby CP, Maekawa T, Sakthikumar D, Mohanan PV. Nano-bio compatibility of PEGylated reduced graphene oxide on mesenchymal stem cells. 2D Materials. 2017;4(2):25066.https://doi.org/10.1088/2053-1583/aa65c2https://dspace.sctimst.ac.in/handle/123456789/10713Graphene, with its unique physico-chemical properties, has found widespread biomedical application. It is used as a carrier for drug or gene delivery, photothermal therapy, bioimaging, in antibacterial agents and for the development of biosensors. Besides this, graphene has the scope to be used for wound healing, tissue engineering and regenerative medicine. In the present study, polyethylene-glycol-(PEG)ylated reduced graphene oxide (PrGO) was synthesized, characterized, and its interaction with mouse bone marrow mesenchymal stem cells (MSCs) was studied. in vitro cytotoxicity and differentiation study showed PrGO neither induced toxicity nor impaired the differentiation potential of the stem cells. PrGO was effectively internalized by MSCs and distributed throughout the cytoplasm. None of the PrGO was seen in the nucleus. Although it seems to induce increased reactive oxygen species (ROS) production inside the cell, no change in cell proliferation or cellular function was observed. Hence it is recommended that the synthesized PrGO is applicable for tissue engineering, and can also be used as a substrate platform for stem cell culture and differentiation.PEGylated reduced graphene oxide, mesenchymal stem cells, cytotoxicity, apoptosis, reactive oxygen species, mitochondrial membrane potentialArticle