Sreekumar, AMandagini, GSubramanian, SPSankunni, AP2017-03-102017-03-10201334 ,2;166-17910.1080/15321819.2012.699493https://dspace.sctimst.ac.in/handle/123456789/9317Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 +/- 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV=6.4%).Biochemistry & Molecular Biology; Immunology; Medical Laboratory Technology