Megha, KBSwathi, SJoseph, XVandana, UMohanan, PV2022-06-062022-06-062022-05Megha KB, Swathi S, Joseph X, Vandana U, Mohanan PV. Cytocompatibility of Pluronics F-127 on adenocarcinomic human alveolar basal epithelial cells (A549 cells). Environmental Science and Pollution Research. 2022 May doi: 10.1007/s11356-022-20925-4https://doi.org/10.1007/s11356-022-20925-4https://dspace.sctimst.ac.in/handle/123456789/11199Pluronics, due to its high water-soluble and thermoreversible ability, attracted much in biomedical applications. They are mainly utilized in drug delivery, gene therapy, and tissue remodeling. The study aims to explore the cytocompatibility of Pluronics F-127, which has gained much popularity due to its various properties. The cells were exposed to varying concentrations of Pluronics F-127 in A549 cells for 24 h. According to the MTT and neutral red assay, A549 cells displayed dose-dependent cell viability. The cell's morphology was preserved after treatment, as seen in phase-contrast and Giemsa staining. When exposed to PF-127, lysosomal, cytoskeletal, and nuclear integrity were maintained. The percentage of live cells in all the treated groups was more significant than 90%, according to the live/dead flow cytometric analyses. The study identified the cytocompatibility of Pluronics F-127 required for the breakthrough in biomedical applications.Biomedical; Cell viability; Cytocompatibility; Nuclear integrity; Pluronics F-127.Article