Thankam, FGMuthu, J2017-03-102017-03-1020133 ,46;24509-2452010.1039/c3ra43710hhttps://dspace.sctimst.ac.in/handle/123456789/9938Studies on the effect of plasma protein binding on biosynthetic hydrogels on the long term cell viability and infiltration onto the hydrogel scaffolds were carried out. Three hydrogels, PALG-P (PALG-co-PEGDA), PALG-PA (PALG-co-PEGDA-co-AA) and PALG-PB (PALG-co-PEGDA-co-BMA) were prepared using a copolymer of poly(propylene fumarate)-co-alginate (PALG) and cross-linker PEGDA and vinyl monomers. The nature of vinyl monomer largely influences the nature of water (structured bound water/freezing free water) present in the hydrogel and also the adsorption of protein, cell growth and infiltration. The extensively bound structured water as observed with butyl methacrylate based poly(propylene fumarate)-co-alginate-PEGDA hydrogel (PALG-PB) do not favour absorption of proteins and sustain cell growth and infiltration for long duration. Though moderately bound structured water favours absorption of protein moderately as observed with poly(propylene fumarate)-co-alginate-PEGDA hydrogel (PALG-PB), it does not sustain the cell growth. However, the minimally bound structured water favours absorption of protein extensively as observed with acrylic acid based poly(propylene fumarate)-co-alginate-PEGDA hydrogel (PALG-PA) and sustains the cell growth and infiltration for long duration.Chemistry