Digital repository of Sree Chitra Tirunal Institute for Medical Sciences and Technology(SCTIMST), Trivandrum.

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Utility of interphase fluorescent in situ hybridization for diagnosis of brain tumors
(SCTIMST, 2024-04-06) Deepti, AN
Brain tumours are classified as per the 5th edition of the WHO classification of tumours of the central nervous system. This latest edition of CNS tumors incorporates molecular features along with the conventional histology leading to “histomolecular” classification. Interphase Fluorescent in situ hybridization (iFISH) is a useful ancillary technique to the pathologist. It is useful to identify gene deletions, amplifications and rearrangements. Fluorescent-tagged FISH probes are hybridized to formalin-fixed paraffin-embedded sections on glass slides and the nuclei are observed under a fluorescent microscope. iFISH is particularly useful in a resource constrained setting where more advanced aids such as methylation profiling and targeted panels are not readily available. The iFISH technique performed in the pathology department will be discussed and the utility of iFISH in neuro-oncology diagnostics will be illustrated through a case-based discussion.
Epilepsy-associated lesions: Surgical Pathology
(SCTIMST, 2024-04-06) Rajalakshmi, P
Epilepsy is a complex disease and is caused by a variety of lesions that fall under various aetiologies such as malformative, genetic, infectious, metabolic, immune-mediated and neoplastic aetiologies. Patients’ evaluation and management require an interdisciplinary approach with the involvement of clinical assessment, electrophysiology, imaging and pathology. Definitive surgeries are offered to patients with poor response to anti-seizure medications and to those with a focal lesion. As Pathologists, we receive tissue samples from surgical resections of the epileptogenic focus. The most common lesions that are diagnosed histopathologically are hippocampal sclerosis, focal cortical dysplasias and neoplasms. This talk will give an overview of the various lesions that a neuropathologist encounters in the surgical samples of epilepsy-related disorders. How the samples are processed and assessed will be illustrated.
3D printed arrowroot starch-gellan scaffolds for wound healing applications
(International Journal of Biological Macromolecules, 2024-03) Abey, J; Fathah, M; Athira, SV; Joseph, X; Megha, KB; Akash, K; Nigina, G; Mohanan, PV; Baiju, GN
Skin, the largest organ in the body, blocks the entry of environmental pollutants into the system. Any injury to this organ allows infections and other harmful substances into the body. 3D bioprinting, a state-of-the-art technique, is suitable for fabricating cell culture scaffolds to heal chronic wounds rapidly. This study uses starch extracted from Maranta arundinacea (Arrowroot plant) (AS) and gellan gum (GG) to develop a bioink for 3D printing a scaffold capable of hosting animal cells. Field emission scanning electron microscopy (FE-SEM) and X-ray diffraction analysis (XRD) prove that the isolated AS is analogous to commercial starch. The cell culture scaffolds developed are superior to the existing monolayer culture. Infrared microscopy shows the AS-GG interaction and elucidates the mechanism of hydrogel formation. The physicochemical properties of the 3D-printed scaffold are analyzed to check the cell adhesion and growth; SEM images have confirmed that the AS-GG printed scaffold can support cell growth and proliferation, and the MTT assay shows good cell viability. Cell behavioral and migration studies reveal that cells are healthy. Since the scaffold is biocompatible, it can be 3D printed to any shape and structure and will biodegrade in the requisite time.
Giant Intradural Vertebral Artery Aneurysms with Mass Effect
(SCTIMST, 2024-03-30) Adarsh, Anil Kumar
Unbled Cerebral Arteriovenous Malformations on Conservative Followup: Clinico-morphological Features, Natural History and Outcomes
(SCTIMST, 2024-03-30) Adarsh, Anil Kumar
Introduction: ARUBA trial was the first randomised controlled clinical trial assessing the outcomes from medical management with interventional therapy versus medical management alone, in patients diagnosed with an unruptured brain arteriovenous malformations. Detailed long term natural history of unbled untreated arteriovenous malformations and its correlation with clinicomorphological features has not been studied widely, especially from our subcontinent. Moreover, quality of life in these patients on conservative management is often not addressed adequately in many studies. In this study, we aimed to evaluate the mortality rate in unruptured AVM patients, who were managed conservatively on long term follow up, and additionally used a standard questionnaire to assess the QOL in these patients. Evaluation of primary outcome {death from any cause or symptomatic stroke (haemorrhage or infarction)} and secondary outcomes {neurological disability (Modified Rankin Scale ≥2) and other neurological symptoms} on long term followup have also not been extensively evaluated.