SCTIMST DSpace

Digital repository of Sree Chitra Tirunal Institute for Medical Sciences and Technology(SCTIMST), Trivandrum.

This repository is for SCTIMST's research, including project reports, theses, publications and more...

Photo by @inspiredimages
 

Communities in DSpace

Select a community to browse its collections.

Now showing 1 - 5 of 5

Recent Submissions

Item
Fabrication and Characterization of Soy Protein/Polyvinyl Alcohol (PVA) Composite Membrane for Guided Tissue Regeneration
(Regenerative Engineering and Translational Medicine, 2024-10) Saranya, CV; Bridget Jeyatha, W; Deepu, DR; Bhatt, A; Lizymol, PP
Purpose Periodontitis is an inflammatory disease that damages the periodontal tissue and leads to tooth loss. Guided tissue regeneration (GTR) is a membrane-based method that prevents the down growth of epithelial and fibroblast cells and gradually restores the periodontal tissues. Currently, collagen membranes exist as the top choice in the field of GTR membranes. However, disease transmission, poor mechanical strength and unpredictable degradation limit its use. The main aim of the study is to fabricate a soy protein–based GTR membrane with good mechanical properties, cell barrier function, and cytocompatibility. Methods Soy protein isolate (SPI) was extracted from the seeds of Glycine max, and the membranes (SPG-1, SPG-2, and SPG-3) were fabricated using SPI, polyvinyl alcohol (PVA), and glycerol (Gly) by aqueous solution casting method. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), contact angle, swelling analysis, and degradation studies of the membranes were carried out. Human periodontal ligament (hPDL) cells were used for the direct contact test, MTT assay, live-dead, cell adhesion, and membrane barrier function experiments. Results SPG-1 membrane exhibited a rough surface and significantly (p ≤ 0.05) lower contact angle (68°) than SPG-3. SPG-1 showed a lower swelling (74.03%) and weight loss percentage (42.13%) (p ≤ 0.001) than SPG-2 and SPG-3. SPG-1 membrane exhibited significantly (p ≤ 0.05) higher tensile strength of 5.7 MPa and suture pull-out strength of 9.04 N when compared with SPG-2 and SPG-3. SPG membranes were non-cytotoxic, cyto-compatible, and prevented the down growth of fibroblast cells. Conclusion SPG-1 membranes with 50% SPI stand out as a best candidate than other SPG membranes with better physiochemical properties. It favoured the growth and proliferation of hPDL cells and exhibited barrier properties. Lay Summary Periodontitis is a disease that affects the structure and function of the periodontal tissues, leading to teeth loss. Guided tissue regeneration (GTR) is a widely accepted treatment using a barrier membrane. Three different composite GTR membranes of soy protein, polyvinyl alcohol, and glycerol were fabricated by the solvent casting method by varying the amount of soy protein isolate. Physiochemical characterization and in vitro studies with human periodontal ligament cells and fibroblast cells demonstrated the suitability of the material for periodontal defect management.
Item
RIGID KNEE BRACE FOR OSTEOARTHRITIS (Project - 7445)
(SCTIMST, 2022-10-20) Subhash, NN; Muraleedharan, CV
Item
Item
Biocompatible InP/ZnSeS quantum dots/MXene composite as highly sensitive electrochemical sensors for carbendazim pesticide
(Applied Surface Science, 2024-11) Sharma, TSK; Jana, J; Sivaselvam, S; Babu, B; Jayasree, RS; Mishra, Y; Hur, SH; Choi, WM
In this study, we prepared InP/ZnSeS QDs anchored on MXene hybrid composite (IZQ-MX) for precise electrochemical sensing of carbendazim (CBZ) pesticide in food and environmental samples. The resulting electrochemical detection of CBZ exhibited a wide linear range of 0.019–527.6 μM, with a low detection limit of 14.59 nM and high sensitivity of 9.9026 µA·µM−1·cm−2. The environmental safety of IZQ-MX composite was further studied using in-vitro and in-vivo model. In L929 cells, exposure to IZQ-MX (0–100 nM) did not induce any significant change in cell viability, intracellular ROS generation and morphological changes. The exposure to IZQ-MX (0–200 nM) in C. elegans has also no significant change in primary and secondary endpoints of nematodes, neuronal development, DAF-16/FOXO and SKN-1/Nrf-2 transcription factors regulating the antioxidant genes. Environmental safety assessment of the IZQ-MX composite was performed using in-vitro and in-vivo models which demonstrated excellent biocompatibility. This work demonstrated the potential of IZQ-MX as significant electrocatalyst in sensitive and selective detection of CBZ for practical applications.
Item
Nitrogen doped carbon quantum dots for bioimaging and detection of norfloxacin residues in food samples
(Journal of Materials Chemistry B, 2024-07) Sivaselvam, S; Anjana, RS; Dhujana, NS; Victor, M; Jayasree, RS
Incomplete metabolism and non-biodegradable nature of norfloxacin (NORx) lead to its persistent residues in the environment and food, potentially fostering the emergence of antibiotic resistance and posing a significant threat to public health. Hence, we developed a norfloxacin sensor employing hydrothermally synthesized N-doped carbon dots (N-Ch-CQDs) from chitosan and PEI demonstrated high sensitivity and specificity towards the antibiotic detection. The quantum yield of excitation-dependent emission of N-Ch-CQDs was effectively tuned from 4.6 to 21.5% by varying the concentration of PEI (5–15%). With the enhanced fluorescence in the presence of norfloxacin, N-Ch-CQDs exhibited a linear detection range of 20–1400 nM with a limit of detection (LoD) of 9.3 nM. The high biocompatibility of N-Ch-CQDs was confirmed in the in vitro and in vivo model and showed the environment-friendly nature of the sensor. Detailed study elucidated the formation of strong hydrogen bonds between N-Ch-CQDs and NORx, leading to fluorescence enhancement. The developed sensor's capability to detect NORx was evaluated in water and milk samples. The recovery rate ranged from 98.5% to 103.5%, demonstrating the sensor's practical applicability. Further, the bioimaging potential of N-Ch-CQDs was demonstrated in both the in vitro (L929 cells) and in vivo model (C. elegans). The synergistic influence of the defecation pattern and functioning of intestinal barrier mitigates the translocation of N-Ch-CQDs into the reproductive organ of nematodes. This study revealed the bioimaging and fluorescent sensing ability of N-Ch-CQDs, which holds significant promise for extensive application in the biomedical field.