Browsing by Author "Ambattu, LA"

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    Collagen synthesis promoting pullulan-PEI-ascorbic acid conjugate as an efficient anti-cancer gene delivery vector
    (CARBOHYDRATE POLYMERS, 2015) Ambattu, LA; Rekha, MR
    Cationized pullulan (pullulan-PEI; PP) was synthesized and further modified with an anti-oxidant molecule, ascorbic acid (PPAA) at various ratios. The nanoplexes formed at an optimum ratio of 4:1 was within a size of 150 nm and had a zeta potential of 9-14 mV. The nanoplexes at this ratio was used for further investigations. The cell internalization and transfection efficiency of these nanoplexes were determined in presence of serum. The internalization and transfection efficiency were found to be unaffected by the presence of fetal bovine serum. Another interesting observation was that this polymer was found to have collagen synthesis promoting property. The collagen synthesis effect of these polymers was quantified and observed that PPAA3 promoted the highest. Transfection efficiency was evaluated by assessing the p53 gene expression in C6 rat glioma cells and cell death was quantified to be 96% by flow cytometry, thus establishing the high efficacy of this polymer. (C) 2015 Elsevier Ltd. All rights reserved.
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    Glutathione-bearing fluorescent polymer-curcumin conjugate enables simultaneous drug delivery and label-free cellular imaging
    (Polymer, 2015-09) Dey, S; Ambattu, LA; Hari, PR; Rekha, MR; Sreenivasan, K
    Curcumin is a natural polyphenol with potential anticancer property. However, the medicinal assets of curcumin cannot be properly utilized due to its low aqueous solubility, instability and subsequent poor bioavailability. Conjugation of curcumin to a suitable hydrophilic polymer is a useful approach for augmenting the aqueous solubility of the drug. In addition, nano-structured materials with intrinsic fluorescence property are of prime importance in the current biomedical domain. In this study, a novel glutathione containing biodegradable, non-toxic and water soluble, fluorescent polymer was developed. Curcumin was covalently conjugated to this polymer to fabricate nano-sized micelle forming fluorescent polymer-curcumin conjugate. The ability of the entity as a safer curcumin delivery vehicle and label-free cellular imaging probe was demonstrated in C6 glioma cells.