Browsing by Author "Anil Kumar, PR"

Now showing 1 - 14 of 14
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    3D printing of liver constructs for in vitro hepatotoxicity testing ( Project - 8141 )
    (SCTIMST, 2021-08-15) Anil Kumar, PR; Shiny, Velayudhan; Roy, Joseph; Kalliyna Krishnan; Kumary, TV; Anugya, Bhatt; Lynda V, Thomas; Sarath S, Nair; Ramesh Babu, V; Sivaram, S; Praveen, KS
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    Alternate adult stem cells for ocular surface regeneration (Project - 8094)
    (SCTIMST, 2017-10) Kumary, TV; Anil Kumar, PR
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    Bioceramic cages with axially aligned pores as a substitute for tricortical bone graft. ( Project - 8222 )
    (SCTIMST, 2020-04-08) Manoj, Komath; Naresh, Kasoju; Harikrishna Varma, PR; Anil Kumar, PR; Anoop, Pillai
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    Corneal Epithelial Cell Sheet Engineering: Validation and Pre-Clinical Evaluation ( Project - 8223 )
    (SCTIMST, 2023-01-20) Naresh, Kasoju; Anil Kumar, PR; Sachin J, Shenoy; Sabareeswaran, A; Chitra, Raghavan,
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    Fabrication of co-cultured tissue constructs using a dual cell seeding compatible cell culture insert with a clip-on scaffold for potential regenerative medicine and toxicological screening application
    (Journal of Science: Advanced Materials and Devices, 2020-06) Ameer, JM; Ramesh Babu, V; Vinod, D; Nishad, KV; Sabareeswaran, A; Anil Kumar, PR; Kasoju, N
    Tissue engineering is emerging as a modern medicine fascination towards the establishment of human tissue banks; yet, these approaches typically involve cultures of only one type of cell and, therefore, do not recapitulate the native tissue physiology in toto. Co-culture models, comprised of different cell types, can potentially create the next level of complexity. However, conventional approaches involving multiple cell types and cell culture inserts do have limitations. To this end, here we demonstrate a novel cell culture insert that allows the use of any custom-made scaffold, free-flow of fluids/gases, dual cell seeding on either sides of the insert, easy stacking of multiple inserts and resizing it to any multi-well plate format as well as culture dishes. To prove the concept, electrospun silk fibroin scaffold was clipped onto the insert and was used for co-culturing of keratinocytes and fibroblast cells. The results indicated a successful fabrication of spatially organized skin tissue constructs having epidermal and dermal equivalent histology. Cell-laden inserts were stacked and used for simulated transportation studies. However, the conditions need further fine-tuning. All together, the results indicated that the novel cell culture insert with silk fibroin scaffold could be used as a facile, versatile and scalable approach to fabricate and transport 3D co-cultured tissue constructs in vitro, including but not limited to skin. The resultant tissue constructs can be explored for therapeutic applications, for instance as artificial skin substitute in wound healing, and for toxicological applications, for instance as reconstructed skin tissue model in skin irritation testing.
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    Hydroxyapatite cages with aligned pores for bone grafting – Seeding of human osteoblast-like cells in vitro and their response in dynamic culture mode
    (Ceramics International, 2021-11) Athira, RK; Gayathry, G; Anil Kumar, PR; Harikrishna Varma, PR; Kasoju, N; Manoj, Komath
    Hydroxyapatite (HA) is a highly regarded synthetic bone graft material. Porous HA ceramics blocks are used to substitute harvested natural bone grafts. Being similar to bone mineral, HA material integrates with the host bone through surface osteointegration and slowly resorb along with the natural bone remodeling process. The blocks in use currently have random and tortuous pore structures. The present work explores the usefulness of cage-like HA ceramic design with end-to-end open pores, with the help of in vitro cell culture methods. Such a structure, on implantation, will take up the blood factors and cells and host the bone remodeling process inside the bulk of the cage, leading to early healing. In the study, HA samples with aligned through-pores were prepared and explored in vitro, with a focus on how the pores host the cells inside and to what level the cells maintain their activity. Human osteoblast-like cells (HOS) were used, at different seeding and culturing approaches. Cell seeding was done through (i) conventional large volume cell suspension, (ii) a confined mini chamber with a limited volume of cell suspension, and (iii) placing a concentrated drop of cell suspension directly on top of the scaffold. The third approach gave the best cell adhesion and proliferation, and hence used for further explorations. A dynamic culture system was designed in-house by bifurcating the cell culture wells using vertical inserts, holding the samples horizontally with their ends open to both sides, and making the media flow across using a rocker platform. The HOS cell adhesion, viability and proliferation were tested in the HA cages, in static and dynamic culture conditions, with conventional porous ceramics as the control. The cell infiltration was deeper and the cell viability over a period of 7 days was significantly higher in dynamic culture conditions in the test samples.
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    Indigenous bone graft expanders for Masquelet Induced Membrane Technique ( Project - 6240 )
    (SCTIMST, 2022-11-11) Lizymol, PP; Francis Boniface, Fernandez; Anil Kumar, PR
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    Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering
    (MedComm – Future Medicine, 2022-09) Gayathry, G; Athira, RK; Anju, MS; Anil Kumar, PR; Harikrishna Varma, PR; Kasoju, N; Komath, M
    Regeneration of bone lost by trauma, diseases and aging, and restoration of its load-bearing function are major clinical challenges. Hydroxyapatite (HA) is a clinically proven scaffold material for bone grafting, but the random-pore structure limits the homing of the cells inside the graft and the bone regeneration progresses with the resorption of the graft material. This work is based on the hypothesis that aligned through pores in the graft will lead to a faster healing by homing the local cells inside and provide a better environment for new bone formation through the graft structure. The investigation was done using aligned porous HA scaffolds seeded with human Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs) and cultured in a multiwell format bioreactor setup. The cell adhesion was studied by microscopy, cell proliferation was evaluated by Alamar blue assay and osteogenic differentiation was confirmed by biochemical and molecular assays. The results indicate that the hWJ-MSCs infiltrated through the aligned porous network of the scaffold, proliferated well when cultured in the expansion medium, and differentiated into osteogenic lineage when cultured in the differentiation medium.
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    Preparation and standardization of a batch of Reference Biomaterials (RM) for biological evaluations ( Project - 8169 )
    (SCTIMST, 2020-11-27) Leena, Joseph; Ramesh, P; Remya, NS; Ramesh Babu, V; Anil Kumar, PR; Anugya, Bhatt; Sabareeswaran, A
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    Stem Cell Derived Exosome Therapy for Clinical Management of Lung Damage in Critically-ill Corona Viral Pneumonia Patients ( Project - 8194 )
    (SCTIMST, 2021-12-30) Naresh, Kasoju; Senthilkumar, Mutusamy; Harikrishnan, VS; Sabareeswaran, A; Anil Kumar, PR; Francis B, Fernandez
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    A versatile approach for temporary storage and shipping of in vitro cultured cells, cell sheets and tissue engineered constructs – a preliminary report
    (Engineered Regeneration, 2022-09) Anju, MS; Athira, RK; Ramesh Babu, V; Anil Kumar, PR; Kasoju, N
    Temporary storage/ shipping of cell/ tissue engineering products from bench to bedside is a key aspect of regenerative medicine. The current proof-of-concept study presents a multipurpose device for temporary storage/ shipping of cell culture dishes containing cell/ tissue constructs. The device, made with readily available raw materials, contains three elements viz. a specialized lid, polymeric plates having grooves and a set of nuts and bolts. As part of the performance evaluation, the device was first subjected to a simulated storage/ shipping process, wherein the leak-proof and aseptic containment of the contents was demonstrated. Subsequently, the setup was used for temporary storage/ shipping of dishes having (a) L929 cell monolayers cultured on treated surfaces, (b) SIRC, HaCaT and A549 cell sheets cultured on thermo-responsive surfaces, (c) HOS-cell encapsulated agar gels and (d) HOS-cell seeded silk fibroin mats. The results showed that the health of cell monolayers/ cell sheets/ tissue constructs after the process was comparable to that before the process. The device was scalable, simple to handle, can be made for a single or multi-use purpose, and can be resizable to load other culture vessels. The design of the storage/ shipping device described in this report thus offers versatile features and applications.