Browsing by Author "Biby, TE"

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    Effect of polymer functionalized fullerene soot on C6 glial cells
    (European Polymer Journal, 2020-03) Athira, SS; Biby, TE; Mohanan, PV
    Fullerene is crafting its dominance in various fields of science. Advanced exploration on the applicability of fullerene and the enhanced chances of exposure has prompted valuation of its biocompatibility. The antioxidant and radical scavenging characteristic of fullerene raises assurance for various biomedical applications. Nevertheless, the hydrophobicity of fullerene lowers its charm in healthcare applications where stable water dispersity is fundamental. In this study, one of the highly exposed forms of fullerenes; fullerene soot was subjected to in vitro toxicity evaluation. For nullify hydrophobic consistency, soot nanoparticles were surface functionalized with dextran polymer. Even though various tissue-specific toxicity evaluations of fullerene are been reported, the idea about its effect on the neuronal tissue still remains vague. In the context, the well-characterized dextran-coated fullerene soot (Dex-FS) nanoparticles were exposed to C6 glial cells to understand the cellular response of Dex-FS. Glial cells are present in plenty in the central nervous system (CNS) and provide support and insulation to neurons. Hence, glial cells turn out to be an ideal choice for in vitro CNS system. Dex-FS showed dose-dependent toxicity. The integrity of cytoskeleton was found to be compromised. Dex-FS was found to trigger ROS production in a dose and time dependent manner. The lysosomal activity and mitochondrial membrane potential during post Dex-FS treatment was evaluated using acridine orange and rhodamine-phalloidin staining. The molecular level toxicity was evaluated by DNA laddering and DAPI staining. The amount of live and dead cells after treating C6 glial cells with Dex-FS was estimated by Calcein AM/ PI flow cytometry. The neurotoxic impact of fullerene soot nanoparticles explored in the present study thus presents a debatable topic regarding vehicle exhaust which contains higher amounts of fullerene soot as active ingredient.
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    Toxicity of dextran stabilized fullerene C60 against C6 Glial cells
    (Brain Research Bulletin, 2020-01) Biby, TE; Prajitha, N; Ashtami, J; Sakthikumar, D; Maekawa, T; Mohanan, PV
    Elevated application potential of fullerene C60 paved the way to think on its adverse effect when it reaches to biological system and environment. Though fullerenes are insoluble in water, various strategies are employed to make it soluble. Method of solubilization with organic solvents, yield cytotoxic responses both in vitro and in vivo. In this study, dextran was used to stabilize C60 particle. Fourier transformed-infrared spectroscopy (FT-IR) and transition electron microscopy (TEM) were used for characterization and it confirms effective surface stabilization and morphological characteristics. This was followed by various cytotoxicity studies to evaluate its bio-nano interactions. The results of the study suggest that the dextran stabilized C60 nanoparticles (Dex-C60) forms uniform suspension in water and was stable up to 72 h. The C6 glial cell-Dex-C60 interactions indicated that the Dex-C60 nanoparticles penetrate deeper into the cells and cause dose dependent toxic response. The result of the study recommended that Dex-C60 nanoparticles should undergo intensive risk assessment before biomedical applications and should take proper safety measure to avoid its entry to the environment.