Browsing by Author "Deepa, S"

Now showing 1 - 5 of 5
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    A preliminary study on monocyte-platelet interactions in diabetic subjects leading to phenotypic changes in monocytes
    (Indian Journal of Applied Research, 2020-07) Deepa, S; Bhatt, A
    Background and objectives: Platelets become hyperactive during progression of inflammatory disease conditions such as diabetes mellitus. We aimed to assess whether activated platelets (CD62+) interact with monocytes to form monocyteplatelet aggregates and such aggregates change the phenotype of monocytes into proinflammatory phenotype (CD14-CD16+) via a comparative study between diabetic and non diabetic subjects. Methods: Based on inclusion and exclusion criteria, study subjects were chosen. Monocytes isolated from buffy coat were characterised using immunostaining and flow cytometry. Phenotypic changes in monocytes were determined through flow cytometry; interaction between monocytes and platelets were determined through ow cytometry, ESEM. Proteomic studies were carried out to determine inflammatory marker expression. Results: Study revealed that there are monocyte-platelet interactions and alteration in monocyte phenotypes occurring in diabetic subjects compared to control subjects. Western blotting analysis showed the expression of inflammatory molecules IL-10 and MCP-1 in diabetic subjects, further confirming the proinflammatory nature of monocytes. Conclusion: Platelet monocytes interaction in diabetic subjects may lead to the phenotypic alteration in monocytes. Inflammatory protein analysis and cell surface marker analysis of monocytes may be considered as a preliminary tool for assessing complications during diabetes mellitus.
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    Septal Course of Left Anterior Descending Artery From the Right Aortic Sinus in Tetralogy of Fallot: A Benign Anomaly and Important Lessons Learned
    (PEDIATRIC CARDIOLOGY, 2013) Deepa, S; Bijulal, S; Baiju, DS; Thomas, M
    The proximal course of an anomalously arising coronary artery is a decisive factor in the surgical approach for tetralogy of Fallot (TOF). Studies have shown that echocardiography provides a good anatomic definition of the ostium and proximal epicardial course of coronary arteries [1, 2]. This report describes a case of TOF with an atrioventricular canal defect whose preoperative echocardiography showed abnormal origin of the left anterior descending artery (LAD) from right aortic sinus, which was interpreted as crossing the right ventricular outflow tract. Perioperative inspection did not show any abnormal vessel crossing the outflow, and corrective surgery was performed. At the echocardiographic evaluation after surgery, it was noted that the abnormal LAD arising from right aortic sinus was taking a septal course in relation to the posterior aspect of the pulmonary annulus. It is important to recognize this anomalous course because it is benign with no surgical implications.