Browsing by Author "Durgadas, CV"

Now showing 1 - 3 of 3
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    Aggregation of gold nanoparticles followed by methotrexate release enables Raman imaging of drug delivery
    (J Nanoparticle Res., 2012-10) Durgadas, CV; Sharma, CP; Paul, W; Rekha, MR; Sreenivasan, K
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    Aggregation of gold nanoparticles followed by methotrexate release enables Raman imaging of drug delivery into cancer cells
    (JOURNAL OF NANOPARTICLE RESEARCH, 2012) Durgadas, CV; Sharma, CP; Paul, W; Rekha, MR; Sreenivasan, K
    This study refers an aqueous synthesis of methotrexate (MTX)-conjugated gold nanoparticles (GNPs), their interaction with HepG2 cells, and the use of Raman imaging to observe cellular internalization and drug delivery. GNPs of average size 3.5-5 nm were stabilized using the amine terminated bifunctional biocompatible copolymer and amended by conjugating MTX, an anticancer drug. The nanoparticles were released MTX at a faster rate in acidic pH and subsequently found to form aggregates. The Raman signals of cellular components were found to be enhanced by the aggregated particles enabling the mapping to visualize site-specific drug delivery. The methodology seems to have potential in optimizing the characteristics of nanodrug carriers for emptying the cargo precisely at specified sites.
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    Sensing of lead ions using glutathione mediated end to end assembled gold nanorod chains
    (SENSORS AND ACTUATORS B-CHEMICAL, 2011) Durgadas, CV; Lakshmi, VN; Sharma, CP; Sreenivasan, K
    This communication reports the use of glutathione mediated self assembled chains of gold nanorods as new approach for the detection of Pb(2+) ions. We were prompted to study the influence of metal ions by considering the role of glutathione as detoxification agent in the body. Additionally the ability of glutathione to complex with metal ions like lead (Pb(2+)) and mercury (Hg(2+)) is well known. We studied the interaction of different metal ions including Pb(2+) with the end to end assembled chains of gold nanorods. Pb(2+) was found to disassemble the chains to dimeric structures. High resolution transmission electron microscopy and dynamic light scattering were used to study the ensemble. A proportional reduction in the size of the assembly was observed between concentration ranges of 0.1-0.025 mM of Pb(2+). Our results indicate that analyte induced disassembly is an attractive approach to the detection of environmentally relevant components such as Pb(2+). (C) 2011 Elsevier B.V. All rights reserved.