Browsing by Author "Finosh, GT"

Now showing 1 - 4 of 4
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    Hybrid alginate-polyester bimodal network hydrogel for tissue engineering - Influence of structured water on long-term cellular growth
    (COLLOIDS AND SURFACES B-BIOINTERFACES, 2015) Finosh, GT; Jayabalan, M; Vandana, S; Raghu, KG
    The development of biodegradable scaffolds (which promote cell-binding, proliferation, long-term cell viability and required biomechanical stability) for cardiac tissue engineering is a challenge. In this study, biosynthetic amphiphilic hybrid hydrogels were prepared using a graft comacromer of natural polysaccharide alginate and synthetic polyester polypropylene fumarate (PPF). Monomodal network hydrogel (HPAS-NO) and bimodal network hydrogel (HPAS-AA) were prepared. Between the two hydrogels, HPAS-AA hydrogel excels over the HPAS-NO hydrogel. HPAS-AA hydrogel is mechanically more stable in the culture medium and undergoes gradual degradation in vitro in PBS (phosphate buffered saline). HPAS-AA contains nano-porous structure and acquires structured water (non-freezing-bound water) (53.457%) along with free water (11.773%). It absorbs more plasma proteins and prevents platelet adsorption and hemolysis when contacted with blood. HPAS-AA hydrogel is cytocompatible and promote 3D cell growth (approximate to 170%) of L929 fibroblast even after 18 days and H9C2 cardiomyoblasts. The enhanced and long-term cellular growth of HPAS-AA hydrogel is attributed to the cell responsive features of structured water. HPAS-AA hydrogel can be a better candidate for cardiac tissue engineering applications. (c) 2015 Elsevier B.V. All rights reserved.
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    Hybrid amphiphilic bimodal hydrogels having mechanical and biological recognition characteristics for cardiac tissue engineering
    (RSC ADVANCES, 2015) Finosh, GT; Jayabalan, M
    Tissue engineering strategies rely on the favourable microniche scaffolds for 3D cell growth. For cardiac tissue engineering, a biodegradable hydrogel has to meet the essential requirements viz. adequate strength, compatibility of degradation products to the host tissue, maintenance of cellular viability and differentiation, favouring cell integration, controlled degradation of scaffold commensurate with the contractile function under ischemic conditions of the injured heart. In this work, an attempt is made to explore some of these stringent and diagonally opposite requirements. Hybrid amphiphilic bimodal hydrogels having mechanical and biological recognition characteristics were developed using graft comacromer of alginate-poly(mannitol fumarate-co-sebacate). Alginate was graft copolymerized with poly(mannitol fumarate-co-sebacate) macromer (HT-MFS). The resultant comacromer was crosslinked with PEGDA and DEGDMA to form two bimodal hydrogel scaffolds. Both hydrogels exhibited better physiochemical and mechanical properties and supported long-term cell viability under static and dynamic conditions. Laser scanning confocal microscopy Z-stacking evaluations showed infiltration of FDA-stained L929 fibroblasts in the interstices of the hydrogels with appreciable depth. The hydrogel based on PEGDA promoted cell growth to an extent of 98 mm when compared to that of DEGDMA based hydrogel with 52 mm. These hydrogels supported the co-culture of fibroblasts and cardiomyoblasts and provided a better microniche for the cells as evident by the viability and cell cycle progression analyses. The favourable cellular responses of these hydrogels are attributed to the inherent biological recognition characteristics. On comparing the two hydrogels, the PEGDA-based hydrogel was superior to its DEGDMA counterpart due to the higher hydrophilicity of the former. The PEGDA-based hydrogel is a promising candidate for cardiac tissue engineering.