Browsing by Author "Harikrishna Varma, PR"

Now showing 1 - 7 of 7
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    Apatitic calcium phosphate Bone cements ( Project - 7021 )
    (SCTIMST, 2022-03-31) Manoj, Komath; Harikrishna Varma, PR
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    Asialoglycoprotein receptor targeted optical and magnetic resonance imaging and therapy of liver fbrosis using pullulan stabilized multi-functional iron oxide nanoprobe
    (Scientific Reports., 2021-09) Saraswathy, A; Nazeer, SS; Nimi, N; Hema, S; Parvathy, RS; Jibin, K; Victor, M; Fernandez, FB; Sabareeswaran, A; Shenoy, SJ; Harikrishna Varma, PR; Jayasre, RS
    Early diagnosis and therapy of liver fibrosis is of utmost importance, especially considering the increased incidence of alcoholic and non-alcoholic liver syndromes. In this work, a systematic study is reported to develop a dual function and biocompatible nanoprobe for liver specific diagnostic and therapeutic applications. A polysaccharide polymer, pullulan stabilized iron oxide nanoparticle (P-SPIONs) enabled high liver specificity via asialogycoprotein receptor mediation. Longitudinal and transverse magnetic relaxation rates of 2.15 and 146.91 mM−1 s−1 respectively and a size of 12 nm, confirmed the T2 weighted magnetic resonance imaging (MRI) efficacy of P-SPIONs. A current of 400A on 5 mg/ml of P-SPIONs raised the temperature above 50 °C, to facilitate effective hyperthermia. Finally, a NIR dye conjugation facilitated targeted dual imaging in liver fibrosis models, in vivo, with favourable histopathological results and recommends its use in early stage diagnosis using MRI and optical imaging, and subsequent therapy using hyperthermia.
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    Bioceramic cages with axially aligned pores as a substitute for tricortical bone graft. ( Project - 8222 )
    (SCTIMST, 2020-04-08) Manoj, Komath; Naresh, Kasoju; Harikrishna Varma, PR; Anil Kumar, PR; Anoop, Pillai
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    Development of calcium sulfate based injectable bone substitute. ( Project - 8036 )
    (SCTIMST, 2012-12-24) Manoj, Komath; Harikrishna Varma, PR
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    Hydroxyapatite cages with aligned pores for bone grafting – Seeding of human osteoblast-like cells in vitro and their response in dynamic culture mode
    (Ceramics International, 2021-11) Athira, RK; Gayathry, G; Anil Kumar, PR; Harikrishna Varma, PR; Kasoju, N; Manoj, Komath
    Hydroxyapatite (HA) is a highly regarded synthetic bone graft material. Porous HA ceramics blocks are used to substitute harvested natural bone grafts. Being similar to bone mineral, HA material integrates with the host bone through surface osteointegration and slowly resorb along with the natural bone remodeling process. The blocks in use currently have random and tortuous pore structures. The present work explores the usefulness of cage-like HA ceramic design with end-to-end open pores, with the help of in vitro cell culture methods. Such a structure, on implantation, will take up the blood factors and cells and host the bone remodeling process inside the bulk of the cage, leading to early healing. In the study, HA samples with aligned through-pores were prepared and explored in vitro, with a focus on how the pores host the cells inside and to what level the cells maintain their activity. Human osteoblast-like cells (HOS) were used, at different seeding and culturing approaches. Cell seeding was done through (i) conventional large volume cell suspension, (ii) a confined mini chamber with a limited volume of cell suspension, and (iii) placing a concentrated drop of cell suspension directly on top of the scaffold. The third approach gave the best cell adhesion and proliferation, and hence used for further explorations. A dynamic culture system was designed in-house by bifurcating the cell culture wells using vertical inserts, holding the samples horizontally with their ends open to both sides, and making the media flow across using a rocker platform. The HOS cell adhesion, viability and proliferation were tested in the HA cages, in static and dynamic culture conditions, with conventional porous ceramics as the control. The cell infiltration was deeper and the cell viability over a period of 7 days was significantly higher in dynamic culture conditions in the test samples.
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    Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering
    (MedComm – Future Medicine, 2022-09) Gayathry, G; Athira, RK; Anju, MS; Anil Kumar, PR; Harikrishna Varma, PR; Kasoju, N; Komath, M
    Regeneration of bone lost by trauma, diseases and aging, and restoration of its load-bearing function are major clinical challenges. Hydroxyapatite (HA) is a clinically proven scaffold material for bone grafting, but the random-pore structure limits the homing of the cells inside the graft and the bone regeneration progresses with the resorption of the graft material. This work is based on the hypothesis that aligned through pores in the graft will lead to a faster healing by homing the local cells inside and provide a better environment for new bone formation through the graft structure. The investigation was done using aligned porous HA scaffolds seeded with human Wharton's jelly-derived mesenchymal stem cells (hWJ-MSCs) and cultured in a multiwell format bioreactor setup. The cell adhesion was studied by microscopy, cell proliferation was evaluated by Alamar blue assay and osteogenic differentiation was confirmed by biochemical and molecular assays. The results indicate that the hWJ-MSCs infiltrated through the aligned porous network of the scaffold, proliferated well when cultured in the expansion medium, and differentiated into osteogenic lineage when cultured in the differentiation medium.
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    Tissue-engineered ceramic for promoting osteointegration in osteoporotic animal models with relevance to the clinical problem in women ( Project - 8103 )
    (SCTIMST, 2018-05-13) Annie, John; Harikrishna Varma, PR; Sachin J, Shenoy; Harikrishnan, VS