Browsing by Author "Jaison, PL"

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    Separation of bovine heart galactose lectin from endogenous glycoproteins co-purified with the lectin during affinity chromatography
    (JOURNAL OF BIOSCIENCES, 1998) Appukuttan, PS; Annamma, KI; Geetha, M; Jaison, PL
    During affinity chromatographic purification of bovine heart 14 kDa galactose-binding lectin (galectin 1) on lactose-Sepharose, several high molecular weight non-lectin glycoproteins were co-purified with the lectin. Glycoprotein binding to the affinity matrix was neither hydrophobic nor ionic, but galactose-dependent since lactose abolished binding. Purification of galectin from the co-purified glycoproteins by affinity electrophoresis in presence of the specific sugar lactose increased agglutination activity about 65-fold, indicating that a complex containing galectin molecules bound sugar specifically to endogenous glycoproteins with sugar binding sites still available had been retained on lactose-Sepharose.