Browsing by Author "Joseph, Josna"

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    Investigative Study of Myofibroblasts and Cytokines in Peri-Implant Tissue of Silicone Breast Expander by RT-PCR in a Rat Model
    (JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2010)
    The excessive collagen deposition around silicone breast implants followed by contracture and development of severe pain is a major clinical problem. This study was conducted to investigate the profibrotic and antifibrotic cytokines secreted by inflammatory cells and development of myofibroblasts at the tissue material interface around silicone breast expander and ultra-high-molecular-weight polyethylene (UHMWPE). Both materials were implanted in rats for 30, 90 and 180 days. Inflammatory cells and collagen deposition at the material-tissue interface were assessed with Haematoxylin-Eosin and Masson's Trichrome stain. Gene expression of TGF beta, IL-1 beta, IFN gamma, IL10 and alpha-SMA was quantitated by real-time (RT)-PCR in the peri-implant tissue. Results indicate a difference in collagen deposition and myofibroblast development around both materials with involvement of TGF beta, IFN gamma and IL10. The results emphasise the need for further investigation into the molecular mechanisms of protomyofibroblast and myofibroblast formation around silicone implants, which would provide information on these target cells for inhibitory therapy in the clinical situation. (C) Koninklijke Brill NV, Leiden, 2010
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    Role of immune cells and inflammatory cytokines in regulation of fibrosis around silicone expander implants
    (JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2010)
    This study aimed to investigate the progress of wound healing around silicone expander with particular emphasis on fibroblasts, myofibroblasts and collagen in the repair phase. Semi-quantitative evaluation of inflammatory cells and their cytokines, fibroblasts and myofibroblasts at the tissue-material interface was carried out. Commercially available silicone expander was implanted in gluteus muscle of young female Wistar rats for 3, 7, 14, 30, 90 and 180 days. Ultra high molecular weight polyethylene served as control. The cellular response was studied by immunohistochemistry and Transmission Electron Microscopy. A thick collagenous fibrous capsule was observed around the silicone expander at 180 days, with persistent myofibroblasts, lymphocytes and macrophages as compared to the thin fibrous encapsulation around the UHMWPE implants. The regulatory role of cytokines and immune cells in myofibroblast persistence in tissue-implant interface around silicone expander has been extensively studied. Results of this study indicate the need to elucidate the signaling molecules in the transition of fibroblast to myofibroblast around silicone expander implants.