Browsing by Author "Kumar, BS"

Now showing 1 - 4 of 4
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    Guillain-Barre syndrome occurring as a complication of acute nonlymphoblastic leukemia.
    (AMERICAN JOURNAL OF MEDICINE, 1999) Geetha, N; Hussain, BM; Lali, VS; Nair, MK; Kumar, BS
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    Influence of Tissue Fluorescence Measurement and Imaging by Auto-Fluorescence of Substrata
    (JOURNAL OF APPLIED SPECTROSCOPY, 2015) Kumar, BS; Sandhyamani, S; Nazeer, SS; Jayasree, RS
    Polymers, glass and fiber reinforced materials are increasingly being used in photophysical devices. The inherent fluorescence of devices made of these materials may itself become important during fluorescence detection. It may interfere with or even negate the low fluorescence signals emitted from fluorophores molecules of interest especially when the fluorophores exist in microvolume samples. Such effects are significant when tissue micro-sections placed on substrata of high background fluorescence. Using different excitation wavelengths, fluorescence spectral and imaging studies were carried out to examine the effect of background fluorescence of microslides made of various substrata such as glass, polycarbonate and aluminium on autofluorescence of tissue sections. With decreasing wavelength of excitation, all substrata showed increased autofluorescence. Glass showed least inherent fluorescence and aluminium showed more reflectance than autofluorescence. After continuous exposure to blue and UV light, all substrata showed irreversible decreased autofluorescence. During spectrofluorimetric studies, a definite blue shift was observed in the autofluorescence of tissue sections placed on microslides made of each of the materials. This suggests an interference of tissue autofluorescence by background fluorescence emitted by the substratum. This may lead to false positive or negative reporting of the fluorophores, particularly important while analyzing micro-sections of biological samples. Microscopic imaging did not show any background fluorescence for glass substratum with blue light. However, with UV light, glass also showed background fluorescence during imaging. Other substrata showed strong background fluorescence or reflectance with both blue and UV light. This study has the potential for accurate quantification of fluorescence spectral and decay and improved fluorescence imaging using the photobleaching effect.
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    Poorly differentiated carcinoma of the lung presenting with Lambert-Eaton myasthenic syndrome
    (AMERICAN JOURNAL OF CLINICAL ONCOLOGY-CANCER CLINICAL TRIALS, 2000) Nair, SG; Kumar, BS; Rajan, B
    Lambert-Eaton myasthenic syndrome commonly seen in small-cell lung cancer represents an autoimmune reaction against antigens coexpressed by tumor and neurons. It is rarely seen with other histologic subtypes. Symptoms antedate the appearance of the neoplasm by weeks to years. Therapeutic options range from immunosuppression, plasmapheresis, pharmacologic facilitation of neuromuscular transmission, and definitive therapy of the primary tumor. This case report describes the rare association of Lambert-Eaton myasthenic syndrome with non-small-cell lung cancer.
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    Rapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections
    (INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 2015) Kumar, BS; Sandhyamani, S; Nazeer, SS; Jayasree, RS
    Autofluorescence exhibited by tissues often interferes with immunofluorescence. Using imaging and spectral analysis, we observed remarkable reduction of autofluorescence of formalin fixed paraffin embedded tissues irradiated with light prior to incubation with immunofluorescent dyes. The technique of photobleaching offers significant improvement in the quality and specificity of immunofluorescence. This has the potential for better techniques for disease diagnosis.