Browsing by Author "Mayuri, PV"

Now showing 1 - 4 of 4
  • Item
    Effect of photografting 2-hydroxyethyl acrylate on the hemocompatibility of electrospun poly(ethylene-co-vinyl alcohol) fibroporous mats
    (MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2016) Mayuri, PV; Bhatt, A; Joseph, R; Ramesh, P
    Poly(ethylene-co-vinyl alcohol) (EVAL) has been recommended as a material suitable for blood contacting applications. Effect of ethylene content and associated hydrophobicity of EVAL on the blood-material interactions have been documented in the literature. In this work, surface chemistry of EVAL substrate was altered by photografting a hydrophilic monomer, 2-hydroxyethyl acrylate (HEA) with the aid of a photoinitiator, benzophenone (BP), and the effect of surface modification on the blood-material interactions was investigated. Since the modified material was intended to be used as leukodepletion filters, a solution containing EVAL, HEA and BP was electrospun into fibroporous mats and UV treated to induce grafting. Degree of grafting, bonding between fibers and fiber diameter increased with increase in UV exposure time whereas mechanical properties showed a decreasing trend. Decreased water contact angle indicated improved wetting characteristics. In vitro hemocompatibility tests revealed that the modified EVAL surface exhibited significantly lower hemolytic activity, protein adsorption and platelet adhesion than neat EVAL. The modification did not have any substantial effect on the activation of the complement system and coagulation parameters. Photografting led to significant reduction in the adhesion of red blood cells (RBC) whereas white blood cell (WBC) consumption remained above 90%. The results implied that photografting HEA on EVAL substantially improves hemocompatibility of EVAL and when it is used as a filter, it selectively removes leukocytes and allows easy passage of other blood components. (C) 2015 Elsevier B.V. All rights reserved.
  • Item
    Effect of photografting 2-hydroxyethylacrylate on the hemocompatibility of electrospun poly(ethylene-co-vinyl alcohol) fibroporous mats
    (Materials Science and Engineering, 2015-11) Mayuri, PV; Bhatt, A; Joseph, R; Ramesh, P
    Poly(ethylene-co-vinyl alcohol) (EVAL) has been recommended as a material suitable for blood contacting applications. Effect of ethylene content and associated hydrophobicity of EVAL on the blood–material interactions have been documented in the literature. In this work, surface chemistry of EVAL substrate was altered by photografting a hydrophilic monomer, 2-hydroxyethyl acrylate (HEA) with the aid of a photoinitiator, benzophenone (BP), and the effect of surface modification on the blood–material interactions was investigated. Since the modified material was intended to be used as leukodepletion filters, a solution containing EVAL, HEA and BP was electrospun into fibroporous mats and UV treated to induce grafting. Degree of grafting, bonding between fibers and fiber diameter increased with increase in UV exposure time whereas mechanical properties showed a decreasing trend. Decreased water contact angle indicated improved wetting characteristics. In vitro hemocompatibility tests revealed that the modified EVAL surface exhibited significantly lower hemolytic activity, protein adsorption and platelet adhesion than neat EVAL. The modification did not have any substantial effect on the activation of the complement system and coagulation parameters. Photografting led to significant reduction in the adhesion of red blood cells (RBC) whereas white blood cell (WBC) consumption remained above 90%. The results implied that photografting HEA on EVAL substantially improves hemocompatibility of EVAL and when it is used as a filter, it selectively removes leukocytes and allows easy passage of other blood components
  • Item
    Fabrication and characterization of silver nanoparticle impregnated uniaxially aligned fibre yarns by one-step electrospinning process
    (Journal of Materials Science, 2016-01) Mayuri, PV; Ramesh, P
    Generating aligned fibres by traditional electrospinning setup is a significant topic which demands serious consideration. A versatile approach for the generation of uniaxially aligned electrospun fibre yarns by simply altering the electrostatic properties of the polymer solution was discussed in this work. Uniaxially aligned electrospun poly(ethylene-co-vinyl alcohol) (EVAL) fibre yarns were fabricated by one-step electrospinning setup. The alignment was accomplished by increasing the solution conductivity by the addition of AgNO3 to the solution. The jet produced was highly stable and followed a straight path, owing to the enhanced solution conductivity. High degree of fibre alignment was obtained even at low surface velocity of the collector. Subsequently the fibre yarns were thermally reduced to obtain silver nanoparticles (Ag NPs) on the surface of the fibres. The so produced Ag NPs incorporated EVAL fibre yarns were characterized by thermogravimetic analysis, energy dispersive X-ray analysis and scanning electron microscopy. The aligned fibre yarns also exhibited excellent mechanical strength. The method could be recommended for the simultaneous generation of aligned and Ag NPs impregnated fibre yarns for broad spectrum of applications.
  • Item
    A novel leukodepletion filter from electrospun poly(ethylene-vinyl alcohol) membranes and evaluation of its efficiency
    (International Journal of Polymeric Materials and Polymeric Biomaterials, 2016-01) Mayuri, PV; Bhatt, A; Sabareeswaran, A; Ramesh, P
    The authors describe the fabrication and evaluation of a novel leukodepletion filter using novel poly (ethylene-vinyl alcohol) membranes through electrospinning. The membranes were biocompatible and nonhemolytic. A prototype of the filter was developed by stacking the membranes in a poly(methyl methacrylate) case. Upon whole blood filtration, the filter could achieve 100% leukodepletion while capturing 8% red blood cells and 91% platelets. The length of filtration through the developed filter was high when compared to that of marketed filter while overall performance of the filter was comparable to that of the marketed filter