Browsing by Author "Naresh, Kasoju"

Now showing 1 - 4 of 4
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    Bioceramic cages with axially aligned pores as a substitute for tricortical bone graft. ( Project - 8222 )
    (SCTIMST, 2020-04-08) Manoj, Komath; Naresh, Kasoju; Harikrishna Varma, PR; Anil Kumar, PR; Anoop, Pillai
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    Corneal Epithelial Cell Sheet Engineering: Validation and Pre-Clinical Evaluation ( Project - 8223 )
    (SCTIMST, 2023-01-20) Naresh, Kasoju; Anil Kumar, PR; Sachin J, Shenoy; Sabareeswaran, A; Chitra, Raghavan,
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    Formulation and Characterization of Alginate Dialdehyde, Gelatin and Platelet Rich Plasma Based Bioink for Bioprinting
    (Applications. Bioengineering, 2020-09) Lakshmi T., Somasekharan; Naresh, Kasoju; Riya, Raju; Anugya, Bhatt
    Layer-by-layer additive manufacturing process has evolved into three-dimensional (3D) “bio-printing” as a means of constructing cell-laden functional tissue equivalents. The process typically involves the mixing of cells of interest with an appropriate hydrogel, termed as “bioink”, followed by printing and tissue maturation. An ideal bioink should have adequate mechanical, rheological, and biological features of the target tissues. However, native extracellular matrix (ECM) is made of an intricate milieu of soluble and non-soluble extracellular factors, and mimicking such a composition is challenging. To this end, here we report the formulation of a multi-component bioink composed of gelatin and alginate -based scaffolding material, as well as a platelet-rich plasma (PRP) suspension, which mimics the insoluble and soluble factors of native ECM respectively. Briefly, sodium alginate was subjected to controlled oxidation to yield alginate dialdehyde (ADA), and was mixed with gelatin and PRP in various volume ratios in the presence of borax. The formulation was systematically characterized for its gelation time, swelling, and water uptake, as well as its morphological, chemical, and rheological properties; furthermore, blood- and cytocompatibility were assessed as per ISO 10993 (International Organization for Standardization). Printability, shape fidelity, and cell-laden printing was evaluated using the RegenHU 3D Discovery bioprinter. The results indicated the successful development of ADA–gelatin–PRP based bioink for 3D bioprinting and biofabrication applications
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    Stem Cell Derived Exosome Therapy for Clinical Management of Lung Damage in Critically-ill Corona Viral Pneumonia Patients ( Project - 8194 )
    (SCTIMST, 2021-12-30) Naresh, Kasoju; Senthilkumar, Mutusamy; Harikrishnan, VS; Sabareeswaran, A; Anil Kumar, PR; Francis B, Fernandez