Browsing by Author "Parvathy, RS"

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    Asialoglycoprotein receptor targeted optical and magnetic resonance imaging and therapy of liver fbrosis using pullulan stabilized multi-functional iron oxide nanoprobe
    (Scientific Reports., 2021-09) Saraswathy, A; Nazeer, SS; Nimi, N; Hema, S; Parvathy, RS; Jibin, K; Victor, M; Fernandez, FB; Sabareeswaran, A; Shenoy, SJ; Harikrishna Varma, PR; Jayasre, RS
    Early diagnosis and therapy of liver fibrosis is of utmost importance, especially considering the increased incidence of alcoholic and non-alcoholic liver syndromes. In this work, a systematic study is reported to develop a dual function and biocompatible nanoprobe for liver specific diagnostic and therapeutic applications. A polysaccharide polymer, pullulan stabilized iron oxide nanoparticle (P-SPIONs) enabled high liver specificity via asialogycoprotein receptor mediation. Longitudinal and transverse magnetic relaxation rates of 2.15 and 146.91 mM−1 s−1 respectively and a size of 12 nm, confirmed the T2 weighted magnetic resonance imaging (MRI) efficacy of P-SPIONs. A current of 400A on 5 mg/ml of P-SPIONs raised the temperature above 50 °C, to facilitate effective hyperthermia. Finally, a NIR dye conjugation facilitated targeted dual imaging in liver fibrosis models, in vivo, with favourable histopathological results and recommends its use in early stage diagnosis using MRI and optical imaging, and subsequent therapy using hyperthermia.
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    A dual signal on-off fluorescent nanosensor for the simultaneous detection of copper and creatinine
    (Materials Science and Engineering : C, 2019-12) Nair, RV; Parvathy, RS; Jayasree, RS
    The transition of conventional medicine to personalized medicine has paved the roadand need for the sensing of newer biomolecules. Hence, this area has attracted wide interestrecently, due to its capacity to provide information on point of care basis. Multi-analytedetection sensors have emerged recently as they can offer the opportunity to perform quickand affordable analysis with minimum blood sample, as compared to traditional sensing ofeach analytes individually. The present study focuses on the development of a quantum dot(Qd) based nanosensor for the simultaneous detection of copper and creatinine; twobiologically relevant molecules. The sensor was designed by forming a complex of Qd withEthyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and picric acid through carboxylic bond formation of Qd-EDC with picric acid. The dual independent emissions of the Qd-EDCcomplex was used for the simultaneous detection of creatinine and copper by a turn on/turnoff method and was successfully demonstrated with a sensitivity of nanomolar to millimolar,and micromolar to millimolar range respectively. The multianalyte sensor thus developed hasquick response and works well under normal conditions of temperature and pH. It is alsoshown to work in cellular environment and blood serum. A mobile App based detection ofcreatinine using the developed sensor strips has also been attempted and validated withhuman blood samples