Browsing by Author "Poulose, AC"

Now showing 1 - 3 of 3
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    Characterizing the biocompatibility and tumor-imaging capability of Cu2S nanocrystals in vivo
    (NANOSCALE, 2015) Poulose, AC; Veeranarayanan, S; Mohamed, MS; Sakamoto, Y; Hirosawa, N; Suzuki, Y; Zhang, MF; Yudasaka, M; Radhakrishnan, N; Maekawa, T; Mohanan, PV; Kumar, DS
    Multifunctional nanomaterial-based probes have had key impacts on high-resolution and high-sensitivity bioimaging and therapeutics. Typically, NIR-absorbing metal sulfide-based nanocrystals (NCs) are highly assuring due to their unique optical properties. Yet, their in vivo behavior remains undetermined, which in turn undermines their potential bioapplications. Herein, we have examined the application of PEGylated Cu2S NCs as tumor contrast optical nanoprobes as well as investigated the short-and long-term in vivo compatibility focusing on anti-oxidant defense mechanism, genetic material, immune system, and vital organs. The studies revealed an overall safe profile of the NCs with no apparent toxicity even at longer exposure periods. The acquired observations culminate into a set of primary safety data of this nano-material and the use of PEGylated Cu2S NCs as promising optical nanoprobes with immense futuristic bioapplications.
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    Characterizing the biocompatibility and tumorimaging capability of Cu2S nanocrystals in vivo
    (Nanoscale, 2015-09) Poulose, AC; Veeranarayanan, V; Mohamed, MS; Sakamoto, Y; Zhang, M; Yudasaka, M; Radhakrishnan, N; Maekawa, T; Mohanan, PV; Kumar, DS
    Multifunctional nanomaterial-based probes have had key impacts on high-resolution and high-sensitivity bioimaging and therapeutics. Typically, NIR-absorbing metal sulfide-based nanocrystals (NCs) are highly assuring due to their unique optical properties. Yet, their in vivo behavior remains undetermined, which in turn undermines their potential bioapplications. Herein, we have examined the application of PEGylated Cu2S NCs as tumor contrast optical nanoprobes as well as investigated the short- and long-term in vivo compatibility focusing on anti-oxidant defense mechanism, genetic material, immune system, and vital organs. The studies revealed an overall safe profile of the NCs with no apparent toxicity even at longer exposure periods. The acquired observations culminate into a set of primary safety data of this nanomaterial and the use of PEGylated Cu2S NCs as promising optical nanoprobes with immense futuristic bioapplications
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    Synthesis And Characterization of Pegylated Reduced Graphene Oxide: Determination of Toxicity Using Bone Marrow Mesenchymal Stem Cells
    (Journal of Applied Chemical Science International., 2015-10) Syama, S; Reshma, S; Cherian, RS; Poulose, AC; Maekawa, TD; Sakthikumar, D; Mohanan, PV
    Graphene is novel class of carbon based nanomaterial that has several unique physico-chemical characteristics. These properties are being exploited in the biomedical field especially stem cell regenerative therapy. Due to their superior mechanical strength, ability to induce stem cell differentiation and proliferation and antibacterial properties, it is used as a coating for tissue engineered scaffolds. However there is a scarcity of literature on biocompatibility of graphene. The aim of this study is to synthesize PEGylated reduced graphene oxide (PrGO) and assess its biocompatibility in bone marrow mesenchymal stem cells (MSCs). PrGO was synthesized by reduction of graphene oxide and characterized using TEM, SAED, AFM, XPS, Raman spectroscopy and FTIR. MSCs were subjected to characterization to evaluate their stemness. The characterized MSCs were exposed to varying concentration of PrGO and MTT assay was carried out. It was found that PEG was successfully coated on to rGO and MSCs maintained their stemness in vitro. Moreover, the PrGO was found to be biocompatible and increase proliferation of MSCs. In conclusion, in-house synthesized PrGO was found to be non toxic when exposed to MSCs. This study can help design PrGO for safe biomedical applications.