Browsing by Author "Resmi, R"

Now showing 1 - 4 of 4
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    Injectable selfcrosslinking hydrogels for meniscal repair: A study with oxidized alginate and gelatin
    (Carbohydrate Polymers, 2020-05) Resmi, R; Parvathy, J; John A, A; Joseph, R
    Injectable in situ gelling hydrogels are viable treatment options for meniscal injuries occurring in athletes. The present study aims to develop an injectable hydrogel via borax complexation of oxidized alginate, followed by a self-crosslinking reaction with gelatin through a Schiff’s base reaction. Gelation kinetics and degree of crosslinking could be controlled by changing the concentration of components and the formation of Schiff ;'s base formation was confirmed by Raman spectroscopy. The injectable alginate dialdehyde-gelatin (15ADA20G) hydrogel showed 423 ± 20 % water uptake, had an average pore size of 48 μm and compressive strength 295 ± 32 kPa. Phase contrast images, scanning electron micrographs and actin staining depicted adhesion, profuse proliferation, and distribution of fibrochondrocytes on the hydrogel demonstrating its cytocompatibility. Application of hydrogel at the pig meniscal tear ex vivo showed good integration with the host meniscal tissue. Further, the histology of 15ADA20G hydrogel filled meniscus showed retention of hydrogel in the close proximity of meniscal tear even after 3days in culture. The self-crosslinking injectable hydrogel offers a niche for the growth of fibrochondrocytes.
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    Scaffold for liver tissue engineering: Exploring the potential of fibrin incorporated alginate dialdehyde-gelatin hydrogel.
    (International J of Biological Macromolecules, 2020-11) Resmi, R; Anusree, KS; Joseph, R; Bhatt, A
    Introduction Development of a tissue-engineered construct for hepatic regeneration remains a challenging task due to the lack of an optimum environment that support the growth of hepatocytes. Hydrogel systems possess many similarities with tissues and have the potential to provide the microenvironment essential for the cells to grow, proliferate, and remain functionally active. Methods In this work, fibrin (FIB) incorporated injectable alginate dialdehyde (ADA) - gelatin (G) hydrogel was explored as a matrix for liver tissue engineering. ADA was prepared by periodate oxidation of sodium alginate. An injectable formulation of ADA-G-FIB hydrogel was prepared and characterized by FTIR spectroscopy, Scanning Electron Microscopy, and Micro-Computed Tomography. HepG2 cells were cultured on the hydrogel system; cellular growth and functions were analyzed using various functional markers. Results FTIR spectra of ADA-G-FIB depicted the formation of Schiff's base at 1608.53 cm−1 with a gelation time of 3 min. ADA-G-FIB depicted a 3D surface topography with a pore size in the range of 100–200 μm. The non-cytotoxic nature of the scaffold was demonstrated using L929 cells and more than 80 % cell viability was observed. Functional analysis of cultured HepG2 cells demonstrated ICG uptake, albumin synthesis, CYP-P450 expression, and ammonia clearance. Conclusion ADA-G-FIB hydrogel can be used as an effective 3D scaffold system for liver tissue engineering.
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    Synthesis and characterization of silver nanoparticle incorporated gelatin-hydroxypropyl methacrylate hydrogels for wound dressing applications
    (JOURNAL OF APPLIED POLYMER SCIENCE, 2017) Resmi, R; Unnikrishnan, S; Krishnan, LK; Krishnan, VK
    A replaceable wound cover which absorbs moisture and resist infection can be used to prevent development of chronic wounds. A major criterion for a replaceable wound dressing is nonadherence to cells to prevent pain upon removal. A major limitation of water absorbing hydrogels used in wound dressing applications is their poor mechanical strength. In this study, gelatin methacrylate (GelMA) was synthesized by reacting Type A porcine skin gelatin with methacrylic anhydride at 50 degrees C. Resultant GelMA monomer containing polyethylene glycol (PEG) protected silver nanoparticles were subsequently copolymerized with 2-hydroxypropyl methacrylate (HPMA) at room temperature by redox mechanism. This resulted in a hydrogel copolymer with optimum mechanical stability and moisture retention while inhibiting microbial contamination and FT-IR spectroscopy was used to confirm copolymer formation. Antimicrobial properties of the hydrogel using agar diffusion showed zone of inhibition against Staphylococcus aureus. Surface morphology was observed using scanning electron microscopy (SEM) and elemental analysis was carried out using energy-dispersive spectroscopy (EDS). Micro-computed tomography (micro-CT) analysis of the hydrogel showed enhancement in the pore size from around 32 m to 48-64 m after incorporation of silver nanoparticles. Degradation of the hydrogel was observed after 48 h when stored in PBS containing collagenase enzyme. In vitro cell culture experiments established absence of cytotoxicity in the hydrogel and nonadherence character to dermal fibroblasts. (C) 2016 Wiley Periodicals, Inc.
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    Synthesis and characterization of silver nanoparticle incorporated gelatinhydroxypropyl methacrylate hydrogels for wound dressing applications
    (Journal of Applied Polymer Science, 2016-12) Resmi, R; Unnikrishnan, S; Krishnan, LK; Krishnan, VK
    A replaceable wound cover which absorbs moisture and resist infection can be used to prevent development of chronic wounds. A major criterion for a replaceable wound dressing is nonadherence to cells to prevent pain upon removal. A major limitation of water absorbing hydrogels used in wound dressing applications is their poor mechanical strength. In this study, gelatin methacrylate (GelMA) was synthesized by reacting Type A porcine skin gelatin with methacrylic anhydride at 50 8C. Resultant GelMA monomer containing polyethylene glycol (PEG) protected silver nanoparticles were subsequently copolymerized with 2-hydroxypropyl methacrylate (HPMA) at room temperature by redox mechanism. This resulted in a hydrogel copolymer with optimum mechanical stability and moisture retention while inhibiting microbial contamination and FT-IR spectroscopy was used to confirm copolymer formation. Antimicrobial properties of the hydrogel using agar diffusion showed zone of inhibition against Staphylococcus aureus. Surface morphology was observed using scanning electron microscopy (SEM) and elemental analysis was carried out using energydispersive spectroscopy (EDS). Micro-computed tomography (micro-CT) analysis of the hydrogel showed enhancement in the pore size from around 32 m to 48–64 m after incorporation of silver nanoparticles. Degradation of the hydrogel was observed after 48 h when stored in PBS containing collagenase enzyme. In vitro cell culture experiments established absence of cytotoxicity in the hydrogel and nonadherence character to dermal fibroblasts