Browsing by Author "Sakamoto, Y"

Now showing 1 - 3 of 3
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    Characterizing the biocompatibility and tumor-imaging capability of Cu2S nanocrystals in vivo
    (NANOSCALE, 2015) Poulose, AC; Veeranarayanan, S; Mohamed, MS; Sakamoto, Y; Hirosawa, N; Suzuki, Y; Zhang, MF; Yudasaka, M; Radhakrishnan, N; Maekawa, T; Mohanan, PV; Kumar, DS
    Multifunctional nanomaterial-based probes have had key impacts on high-resolution and high-sensitivity bioimaging and therapeutics. Typically, NIR-absorbing metal sulfide-based nanocrystals (NCs) are highly assuring due to their unique optical properties. Yet, their in vivo behavior remains undetermined, which in turn undermines their potential bioapplications. Herein, we have examined the application of PEGylated Cu2S NCs as tumor contrast optical nanoprobes as well as investigated the short-and long-term in vivo compatibility focusing on anti-oxidant defense mechanism, genetic material, immune system, and vital organs. The studies revealed an overall safe profile of the NCs with no apparent toxicity even at longer exposure periods. The acquired observations culminate into a set of primary safety data of this nano-material and the use of PEGylated Cu2S NCs as promising optical nanoprobes with immense futuristic bioapplications.
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    Characterizing the biocompatibility and tumorimaging capability of Cu2S nanocrystals in vivo
    (Nanoscale, 2015-09) Poulose, AC; Veeranarayanan, V; Mohamed, MS; Sakamoto, Y; Zhang, M; Yudasaka, M; Radhakrishnan, N; Maekawa, T; Mohanan, PV; Kumar, DS
    Multifunctional nanomaterial-based probes have had key impacts on high-resolution and high-sensitivity bioimaging and therapeutics. Typically, NIR-absorbing metal sulfide-based nanocrystals (NCs) are highly assuring due to their unique optical properties. Yet, their in vivo behavior remains undetermined, which in turn undermines their potential bioapplications. Herein, we have examined the application of PEGylated Cu2S NCs as tumor contrast optical nanoprobes as well as investigated the short- and long-term in vivo compatibility focusing on anti-oxidant defense mechanism, genetic material, immune system, and vital organs. The studies revealed an overall safe profile of the NCs with no apparent toxicity even at longer exposure periods. The acquired observations culminate into a set of primary safety data of this nanomaterial and the use of PEGylated Cu2S NCs as promising optical nanoprobes with immense futuristic bioapplications
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    In vitro evaluation of antioxidant defense mechanism and hemocompatibility of mauran
    (CARBOHYDRATE POLYMERS, 2013) Raveendran, S; Palaninathan, V; Chauhan, N; Sakamoto, Y; Yoshida, Y; Maekawa, T; Mohanan, PV; Kumar, DS
    Mauran (MR), a highly polyanionic sulfated exopolysaccharide was extracted from moderately halophilic bacterium; Halomonas maura and characterized using X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. Purified MR was evaluated for antioxidant defense mechanisms under in vitro conditions using L929, mouse fibroblast cell line and mice liver homogenate. It was demonstrated that MR could impart protective effect against oxidative stress in both cells and tissue up to a concentration of 500 mu g, which is found to be safe under laboratory conditions. Various enzymatic and non-enzymatic parameters of antioxidant mechanisms were evaluated and concluded that MR has the tendency to maintain a balance of antioxidative enzymes with in the test systems studied. Also, hemocompatibility assay performed revealed that MR has a lesser hemolytic index and exhibited a prolonged clotting time, which shows both antihemolytic, and antithrombogenic nature respectively. Furthermore, absorption studies performed using fluorescent-labeled MR confirmed that MR accumulated within the cell cytoplasm neither induced cellular lysis nor affected the cell integrity. (C) 2013 Elsevier Ltd. All rights reserved.