Degradation of Poly(-caprolactone) and bio-interactions with mouse bone marrow mesenchymal stem cells
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Date
2018-01
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Colloids and Surfaces B: Biointerfaces.
Abstract
Bio-inspired scaffolds in bone tissue engineering using multipotential mesenchymal stem cells grow at a
rapidrate foundits successfuluse inorthopedic injury treatment. Poly(-caprolactone)/PCL is widelyused
in medical devices, tissue engineering, and drug delivery systems. Most desirable property of biodegradable
polymer to be employed in medical application is synchronization of degradation with functional
tissue regeneration. Limited studies have incorporated the degradation kinetics and implication of degradation
products of pure unmodified PCL. The present study analyzes shortterm in vitro degradation profile
of PCL films in physiological condition. The study reports weight loss, changes in molecular weight distribution
and morphological variation in PCL thin film over a period of 90-day degradation. When the
degradable material is in contact with host tissue, there exists robust and dynamic microenvironment
controlling the cell functionality. To comprehend the biocompatibility aspects of polymer material, the
study considered mouse bone marrow mesenchymal stem cells (BMSCs) as model system mimicking
in vivo. There was no indication of toxicity revealed with MTT, LDH leakage, direct contact assay and
clonogenic assay. Absence of oxidative stress and apoptosis denotes BMSCs functional integrity sustained
upon exposure to PCL degradation products. Cell cycle analysis and DNA ladder assay confirmed cell survival
and genomic stability. The study revealed that the topography of pure unmodified PCL surface is
suitable for cell adhesion. It was also observed that the viability of differentiated cells (osteoblasts) was
maintained in presence of PCL extract. Furthermore, polymer and its degradation products were proved
to be hemocompatible. These results synergistically suggestthat pure unmodified PCL and its degradation
products are non-toxic at molecular level.
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Keywords
Mesenchymal stem cells Poly(-caprolactone) Degradation Cytotoxicity ROS Hemocompatibility
Citation
Sukanya VS, Mohanan PV. Degradation of Poly(- caprolactone) and bio-interactions with mouse bone marrow mesenchymal stem cells. Colloids and Surfaces B: Biointerfaces. 2018 Mar;163:107-18