Constitution of FibrinBased Niche for In Vitro Differentiation of Adipose-Derived Mesenchymal Stem Cells to Keratinocytes
No Thumbnail Available
Date
2015-04
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
BioResearch Open Access
Abstract
Epithelialization of chronic cutaneous wound is troublesome and may require use of skin/cell substitutes. Adipose-
derived mesenchymal stem cells (ADMSCs) have immense potential as autologous cell source for treating
wounds; they can cross the germ layer boundary of differentiation and regenerate skin. When multipotent adult
stem cells are considered for skin regeneration, lineage committed keratinocytes may be beneficial to prevent
undesirable post-transplantation outcome. This study hypothesized that ADMSCs may be directed to epidermal
lineage in vitro on a specifically designed biomimetic and biodegradable niche. Cells were seeded on the test
niche constituted with fibrin, fibronectin, gelatin, hyaluronic acid, laminin V, platelet growth factor, and epidermal
growth factor in the presence of cell-specific differentiation medium (DM). The ADMSCs grown on bare
tissue culture polystyrene surface in DM is designated DM-control and those grown in basal medium (BM) is
the BM-control. Lineage commitment was monitored with keratinocyte-specific markers such as cytokeratin
14, cytokeratin 5, cytokeratin 19, and integrin a6 at the transcriptional/translational level. The in vitro designed
biomimetic fibrin composite matrix may have potential application as cell transplantation vehicle.
Description
Keywords
growth factor; stem cells; tissue engineering; wounds; regeneration
Citation
Unnikrishnan S, Jayakumar, K, Krishnan LK. Constitution of FibrinBased Niche for In Vitro Differentiation of Adipose-Derived Mesenchymal Stem Cells to Keratinocytes. BioResearch Open Access. 2014 Dec 1;3(6):339-47