A gold nano particle coated porcine cholecyst-derived bio scaffold for cardiac tissue engineering
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Date
2017-06
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Colloids and surfaces: B Biointerfaces
Abstract
Extracellular matrices of xenogeneic origin have been extensively used for biomedical applications,
despite the possibility of heterogeneity in structure. Surface modification of biologically derived biomaterials
using nanoparticles is an emerging strategy for improving topographical homogeneity when
employing these scaffolds for sophisticated tissue engineering applications.Recently, as a tissue engineering
scaffold, cholecyst derived extracellular matrix (C-ECM) has been shown to have several advantages
over extracellular matrices derived from other organs such as jejunum and urinary bladder. This study
explored the possibility of adding gold nanoparticles, which have a large surface area to volume ratio on
C-ECM for achieving homogeneity in surface architecture, a requirement for cardiac tissue engineering.
In the current study, gold nanoparticles (AuNPs) were synthesized and functionalised for conjugating
with a porcine cholecystic extracellular matrix scaffold. The conjugation of nanoparticles to C-ECM
was achieved by 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide/N-hydroxysuccinimide chemistry
and further characterized by Fourier transform infrared spectroscopy, environmental scanning electron
microscopy, energy dispersive X-ray spectroscopy and thermogravimetric analysis. The physical properties
of the modified scaffold were similar to the original C-ECM. Biological properties were evaluated by
using H9c2 cells, a cardiomyoblast cell line commonly used for cellular and molecular studies of cardiac
cells. The modified scaffold was found to be a suitable substrate for the growth and proliferation of the
cardiomyoblasts. Further, the non-cytotoxic nature of the modified scaffold was established by direct
contact cytotoxicity testing and live/dead staining. Thus, the modified C-ECM appears to be a potential
biomaterial for cardiac tissue engineering.
Description
Keywords
Extracellular matrix, Amine functionalisation, Cardiac-patch, Cytotoxicity
Citation
Nair RS, Ameer JM, Alison MR, Anilkumar TV. A gold nano particle coated porcine cholecyst-derived bio scaffold for cardiac tissue engineering. Colloids and surfaces: B Biointerfaces. 2017;157:130-37.